Anti-Cytokeratin 6 KRT6A Antibody

Product Name

Anti-Cytokeratin 6 KRT6A Antibody

View all KRT6A Antibodies

SKU/Catalog Number

A04012-1

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 6 KRT6A Antibody catalog # A04012-1. Tested in WB,ICC/IF,IHC applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cytokeratin 6 KRT6A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04012-1)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

recombinant protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04012-1 is reactive to KRT6A in Human

Applications

A04012-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

60045 MW

Background of KRT6A

Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10 nm diameter filaments. Cytokeratins, which are useful markers of tissue differentiation, also aid in the characterization of malignant tumors. IL-1 and TNFα induce transcription of cytokeratin 6 in epidermal keratinocytes via the C/EBP β transcription factor. In humans, multiple isoforms of cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1,000-1:2,000
ICC: 1:100-1:500
IHC: 1:50-1:200

Validation Images & Assay Conditions

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Western blot analysis of Cytokeratin 6 on human skin lysates using anti-Cytokeratin 6 antibody at 1/1,000 dilution

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ICC staining Cytokeratin 6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Gene/Protein Information For KRT6A (Source: Uniprot.org, NCBI)

Alternative Names

Allergen Hom s 5; CK6A; CK-6A; CK6C; CK6D; CK-6D; cytokeratin 6A; cytokeratin 6C; cytokeratin 6D; Cytokeratin-6A; Cytokeratin-6D; K6A; K6C; K6D; keratin 6A; keratin 6C; keratin 6D; keratin, epidermal type II, K6A; keratin, type II cytoskeletal 6A; Keratin-6A; KRT6C; KRT6D; Type-II keratin Kb6 KRT6A CK-6C, CK-6E, CK6A, CK6C, CK6D, K6A, K6C, K6D, KRT6C, KRT6D, PC3 keratin 6A keratin, type II cytoskeletal 6A|cytokeratin 6A|cytokeratin 6C|cytokeratin 6D|keratin 6A, , type II|keratin 6A, type II|keratin, epidermal type II, K6A|type-II keratin Kb6

For more info on KRT6A, check out the KRT6A Infographic

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In this infographic, you will see the following information for KRT6A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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