Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody

Product Name

Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody

View all KRT6A; KRT6B; KRT6C Antibodies

SKU/Catalog Number

M30943

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody (Catalog # M30943). Tested in IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Cytokeratin 6 (KRT6) (Hyperproliferation-Related Keratin) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M30943)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: KRT6/1702

Isotype

IgG2a, kappa

Immunogen

Recombinant human keratin 6 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M30943 is reactive to KRT6A; KRT6B; KRT6C in Human, Mouse

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

60045 MW

Background of KRT6A; KRT6B; KRT6C

This monoclonal antibody recognizes a protein of 56kDa, identified as cytokeratin 6 (CK6). In humans, multiple isoforms of Cytokeratin 6 (6A-6F), encoded by several highly homologous genes, have distinct tissue expression patterns, and Cytokeratin 6A is the dominant form in epithelial tissue. The gene encoding human Cytokeratin 6A maps to chromosome 12q13, and mutations in this gene are linked to several inheritable hair and skin pathologies. Keratins 6 and 16 are expressed in keratinocytes, which are undergoing rapid turnover in the suprabasal region (also known as hyper-proliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyper-proliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

M30943 is guaranteed for IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For KRT6A; KRT6B; KRT6C (Source: Uniprot.org, NCBI)

Alternative Names

Keratin, type II cytoskeletal 6A;Cytokeratin-6A;CK-6A;Cytokeratin-6D;CK-6D;Keratin-6A;K6A;Type-II keratin Kb6;Hom s 5;KRT6A;K6A, KRT6D;

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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