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Product Info Summary
| SKU: | PA2104 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-14/CASP14 Antibody Picoband®
View all Caspase-14 Antibodies
SKU/Catalog Number
PA2104
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-14/CASP14 Antibody catalog # PA2104. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-14/CASP14 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2104)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Caspase-14, different from the related rat and mouse sequences by four amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2104 is reactive to CASP14 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28 kDa
Calculated molecular weight
27680 MW
Background of Caspase-14
Caspase 14 is an enzyme that in humans is encoded by the CASP14 gene. CASP14 belongs the evolutionarily conserved caspase family. CASP14 was assigned to chromosome 19p13.1. Using Northern blot and semiquantitative RT-PCR analyses, it was showed that CASP14 was transcriptionally upregulated during maintenance of confluent cultures of human epidermal keratinocytes. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier. Caspase 14 seems to play a role in keratinocyte differentiation and cornification and probably regulates maturation of the epidermis by proteolytically processing filaggrin.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2104 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Rat Liver Tissue, Rat Spleen Tissue, A431 Whole Cell, NIH3T3 Whole Cell
IHC: Human Tonsil Tissue, Human Oesophagus Squama Cancer Tissue
Validation Images & Assay Conditions
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Anti-Caspase-14 antibody, PA2104, Western blotting
All lanes: Anti Caspase-14 (PA2104) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: Rat Spleen Tissue Lysate at 50ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: NIH3T3 Whole Cell Lysate at 40ug
Predicted bind size: 28KD
Observed bind size: 28KD
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Anti-Caspase-14 antibody, PA2104, IHC(P)
IHC(P): Human Tonsil Tissue
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Anti-Caspase-14 antibody, PA2104, IHC(P)
IHC(P): Human Oesophagus Squama Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CASP14 (Source: Uniprot.org, NCBI)
Gene Name
CASP14
Full Name
Caspase-14
Weight
27680 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-14;CASP-14;3.4.22.-;Caspase-14 subunit p17, mature form;Caspase-14 subunit p10, mature form;Caspase-14 subunit p20, intermediate form;Caspase-14 subunit p8, intermediate form;CASP14; Casp14|MIC, MICE, mini, mini-ICE|caspase 14|caspase-14|CASP-14
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP14, check out the CASP14 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP14: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-14/CASP14 Antibody Picoband® (PA2104)
Hello CJ!
PA2104 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epigallocatechin-3-gallate (EGCG) inhibits imiquimod-induced psoriasis-like inflammation of BALB/c mice
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Customer Q&As
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7 Customer Q&As for Anti-Caspase-14/CASP14 Antibody Picoband®
Question
We are currently using anti-Caspase-14/CASP14 antibody PA2104 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
The anti-Caspase-14/CASP14 antibody (PA2104) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-17
Question
I see that the anti-Caspase-14/CASP14 antibody PA2104 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-01-08
Question
I have attached the WB image, lot number and protocol we used for keratinocyte using anti-Caspase-14/CASP14 antibody PA2104. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-13
Question
I have a question about product PA2104, anti-Caspase-14/CASP14 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA2104 anti-Caspase-14/CASP14 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-19
Question
Is a blocking peptide available for product anti-Caspase-14/CASP14 antibody (PA2104)?
A. Brown
Verified customer
Asked: 2017-11-24
Answer
We do provide the blocking peptide for product anti-Caspase-14/CASP14 antibody (PA2104). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-11-24
Question
I was wanting to use your anti-Caspase-14/CASP14 antibody for IHC for mouse keratinocyte on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse keratinocyte identification?
B. Jones
Verified customer
Asked: 2014-12-12
Answer
As indicated on the product datasheet, PA2104 anti-Caspase-14/CASP14 antibody has been tested for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse keratinocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-12
Question
Would anti-Caspase-14/CASP14 antibody PA2104 work on feline WB with brain?
R. Krishna
Verified customer
Asked: 2013-04-17
Answer
Our lab technicians have not tested anti-Caspase-14/CASP14 antibody PA2104 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Caspase-14/CASP14 antibody PA2104 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-17
