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Product Info Summary
| SKU: | M06791-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 15 Rabbit Monoclonal Antibody
View all Cytokeratin 15 Antibodies
SKU/Catalog Number
M06791-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 15 Rabbit Monoclonal Antibody catalog # M06791-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 15 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M06791-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DOB-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 15
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M06791-1 is reactive to KRT15 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
49 kDa
Calculated molecular weight
49212 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M06791-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
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Figure 1. Western blot analysis of Cytokeratin 15 using anti-Cytokeratin 15 antibody (M06791-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human A431 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin 15 antigen affinity purified monoclonal antibody (Catalog # M06791-1) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cytokeratin 15 at approximately 49 kDa. The expected band size for Cytokeratin 15 is at 49 kDa.
Click image to see more details
All lanes use the Antibody at 1:4K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For KRT15 (Source: Uniprot.org, NCBI)
Gene Name
KRT15
Full Name
Keratin, type I cytoskeletal 15
Weight
49212 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type I cytoskeletal 15;Cytokeratin-15;CK-15;Keratin-15;K15;KRT15;KRTB; KRT15 CK15, K15, K1CO keratin 15 keratin, type I cytoskeletal 15|CK-15|cytokeratin 15|keratin 15, type I|keratin-15, basic|keratin-15, beta|type I cytoskeletal 15
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT15, check out the KRT15 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT15: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 15 Rabbit Monoclonal Antibody (M06791-1)
Hello CJ!
M06791-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The Role of EGFR/PI3K/Akt/cyclinD1 Signaling Pathway in Acquired Middle Ear Cholesteatoma
Yin D, Tian L, Ye Y, Li K, Wang J, Cheng P, Chen A, Guo F, Huang H. Int J Mol Med. 2012 Apr;29(4):587-92. Doi: 10.3892/Ijmm.2011.871. Epub 2011 Dec 29. Nanog And ??-Catenin: A New Convergence Point In Epsc Proliferation And Differentiation.
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Customer Q&As
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1 Customer Q&As for Anti-Cytokeratin 15 Rabbit Monoclonal Antibody
Question
We are currently using anti-Cytokeratin 15 Rabbit Monoclonal antibody M06791-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on goat tissues as well?
M. Mangal
Verified customer
Asked: 2013-07-25
Answer
The anti-Cytokeratin 15 Rabbit Monoclonal antibody (M06791-1) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-07-25
