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Product Info Summary
| SKU: | M04299 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cytokeratin 13 Rabbit Monoclonal Antibody
View all Cytokeratin 13 Antibodies
SKU/Catalog Number
M04299
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cytokeratin 13 Rabbit Monoclonal Antibody catalog # M04299. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytokeratin 13 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04299)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GAC-11
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cytokeratin 13
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04299 is reactive to KRT13 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
36 kDa
Calculated molecular weight
49588 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04299 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:50000-1:100000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: A431 cell
IHC: human tonsil tissue
Validation Images & Assay Conditions
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Western blot analysis of Cytokeratin 13 expression in A431 cell lysate.
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Immunohistochemical analysis of paraffin-embedded human tonsil, using Cytokeratin 13 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For KRT13 (Source: Uniprot.org, NCBI)
Gene Name
KRT13
Full Name
Keratin, type I cytoskeletal 13
Weight
49588 MW
Superfamily
intermediate filament family
Alternative Names
Keratin, type I cytoskeletal 13;Cytokeratin-13;CK-13;Keratin-13;K13;KRT13; KRT13 CK13, K13, WSN2 keratin 13 keratin, type I cytoskeletal 13|CK-13|cytokeratin 13|keratin 13, type I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KRT13, check out the KRT13 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KRT13: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytokeratin 13 Rabbit Monoclonal Antibody (M04299)
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No publications found for M04299
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Cytokeratin 13 Rabbit Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Cytokeratin 13 Rabbit Monoclonal antibody (M04299)?
Verified Customer
Verified customer
Asked: 2020-02-11
Answer
We do provide the blocking peptide for product anti-Cytokeratin 13 Rabbit Monoclonal antibody (M04299). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-11
Question
I was wanting to use your anti-Cytokeratin 13 Rabbit Monoclonal antibody for WB for mouse tongue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse tongue identification?
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
As indicated on the product datasheet, M04299 anti-Cytokeratin 13 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse tongue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-Cytokeratin 13 Rabbit Monoclonal antibody M04299 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-18
Answer
The anti-Cytokeratin 13 Rabbit Monoclonal antibody (M04299) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-18
Question
Will M04299 anti-Cytokeratin 13 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Lewis
Verified customer
Asked: 2019-06-06
Answer
It shows on the product datasheet, M04299 anti-Cytokeratin 13 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-06
Question
I see that the anti-Cytokeratin 13 Rabbit Monoclonal antibody M04299 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-02-22
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-02-22
