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Product Info Summary
| SKU: | A10436 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Cytochrome P450 39A1 CYP39A1 Antibody
SKU/Catalog Number
A10436
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cytochrome P450 39A1 CYP39A1 Antibody (Catalog # A10436). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cytochrome P450 39A1 CYP39A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10436)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human Cytochrome P450 39A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A10436 is reactive to CYP39A1 in Human, Mouse
Reconstitution
Calculated molecular weight
54116 MW
Background of CYP39A1
Involved in the bile acid metabolism. Has a preference for 24-hydroxycholesterol, and converts it into a 7-alpha-hydroxylated product.
Li-Hawkins J., . Biol. Chem. 275:16543-16549(2000).
The MGC Project Team, ome Res. 14:2121-2127(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10436 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Western blot analysis of extracts from LOVO cells and HUVEC cells, using Cytochrome P450 39A1 antibody A10436.
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Immunofluorescence analysis of A549 cells, using Cytochrome P450 39A1 antibody A10436.
Protein Target Info & Infographic
Gene/Protein Information For CYP39A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP39A1
Full Name
24-hydroxycholesterol 7-alpha-hydroxylase
Weight
54116 MW
Superfamily
cytochrome P450 family
Alternative Names
24-hydroxycholesterol 7-alpha-hydroxylase;1.14.14.26 ;Cytochrome P450 39A1;hCYP39A1;Oxysterol 7-alpha-hydroxylase;CYP39A1; CYP39A1 cytochrome P450 family 39 subfamily A member 1 24-hydroxycholesterol 7-alpha-hydroxylase|cytochrome P450, family 39, subfamily A, polypeptide 1|cytochrome P450, subfamily XXXIX (oxysterol 7 alpha-hydroxylase), polypeptide 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP39A1, check out the CYP39A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP39A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 39A1 CYP39A1 Antibody (A10436)
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No publications found for A10436
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Cytochrome P450 39A1 CYP39A1 Antibody
Question
See attached the WB image, lot number and protocol we used for skin using anti-Cytochrome P450 39A1 antibody A10436. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-01-02
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-02
Question
We are currently using anti-Cytochrome P450 39A1 antibody A10436 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
The anti-Cytochrome P450 39A1 antibody (A10436) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-13
Question
My question regarding product A10436, anti-Cytochrome P450 39A1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-06-20
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A10436 anti-Cytochrome P450 39A1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-06-20
