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Product Info Summary
| SKU: | A08382 |
|---|---|
| Size: | 200ug |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-alpha-Tubulin Tuba1b Antibody
View all Tubulin alpha-1B Antibodies
SKU/Catalog Number
A08382
Size
200ug
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-alpha-Tubulin Tuba1b Antibody (Catalog # A08382). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store Anti-Tubulin Loading Control Antibody at -20°C prior to opening. Aliquot Loading Control Antibody contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge Tubulin Loading Control Antibody if not completely clear after standing at room temperature. This Control Antibody is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-alpha-Tubulin Tuba1b Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08382)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08382 is reactive to TUBA1B in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
10951 MW
Background of Tubulin alpha-1B
Tubulin Loading Control Antibody recognizes microtubules which are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle. This antibody makes an excellent loading control. Anti-Alpha-tubulin antibody is ideal for investigators involved in cell cycle protein research.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08382 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:90,000
IHC: 1:500 - 1:2,000
IF Microscopy: 1:500 - 1:2,000
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
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Western blot analysis of alpha Tubulin expression in whole cell lysates from mouse brain (lane 1), rat brain extract (lane 2), A431 cell lysates (lane 3), Jurkat cell lysates (lane 4) and HeLa whole cell lysates (lane 5) using rabbit anti-alpha-Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A08382) at 1:1,200. Predicted/Observed size: 50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
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Western blot analysis of alpha Tubulin expression in HeLa whole cell lysates (lane 1), NIH/3T3 whole cell lysates (lane 2) using rabbit anti-alpha-Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A08382) at 1:1,000. Predicted/Observed size: 50 kDa, 50 kDa for alpha-Tubulin. Other band(s): N/A.
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ELISA results of rabbit anti-alpha-Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A08382) tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1
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Western blot analysis of alpha Tubulin expression in HEK293 cell lysates (lane 1), HeLa whole cell lysates (lane 2), MCF-7 cell lysates (lane 3), Jurkat cell lysates (lane 4), A431 cell lysates (lane 5), LNCaP cell lysates (lane 6), A-172 cell lysates (lane 7) and NIH/3T3 cell lysates (lane 8) using rabbit anti-alpha-Tubulin Antigen Affinity purified polyclonal antibody (Catalog # A08382) at 1:1,200. Predicted/Observed size: 50 kDa for Alpha-tubulin.
Protein Target Info & Infographic
Gene/Protein Information For TUBA1B (Source: Uniprot.org, NCBI)
Gene Name
TUBA1B
Full Name
Tubulin alpha-1B chain
Weight
10951 MW
Superfamily
tubulin family
Alternative Names
alpha-Tubulin, α-tubulin, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3 TUBA1B K-ALPHA-1 tubulin alpha 1b tubulin alpha-1B chain|alpha tubulin|alpha-tubulin ubiquitous|tubulin K-alpha-1|tubulin alpha-ubiquitous chain|tubulin, alpha, ubiquitous
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TUBA1B, check out the TUBA1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TUBA1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha-Tubulin Tuba1b Antibody (A08382)
Hello CJ!
A08382 has been cited in 14 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CpG Oligodeoxynucleotides Downregulate Placental Adiponectin and Increase Embryo Loss in Non%u2010Obese Diabetic Mice
Validation of Bmi1 as a therapeutic target of hepatocellular carcinoma in mice
Stem cell autocrine CXCL12/CXCR4 stimulates invasion and metastasis of esophageal cancer
Prognostic relevance of melanoma antigen D1 expression in colorectal carcinoma
Baclofen protects primary rat retinal ganglion cells from chemical hypoxia-induced apoptosis through the Akt and PERK pathways
NGFI-B targets mitochondria and induces cardiomyocyte apoptosis in restraint-stressed rats by mediating energy metabolism disorder
Prohibitin protects against oxidative stress-induced cell injury in cultured neonatal cardiomyocyte
Endogenous Nodal promotes melanoma undergoing epithelial-mesenchymal transition via Snail and Slug in vitro and in vivo.
Overexpression of p53 activated by small activating RNA suppresses the growth of human prostate cancer cells
The Signaling Mechanism of TGF-?1 Induced Bovine Mammary Epithelial Cell Apoptosis
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