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Product Info Summary
| SKU: | M00672-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | WB |
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Product info
Product Name
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1)
View all Cytochrome P450 2E1 Antibodies
SKU/Catalog Number
M00672-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) catalog # M00672-2. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00672-2)
Host
Mouse
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Monoclonal
Clone Number
2C7G1
Isotype
Mouse IgG2b
Immunogen
E.coli-derived human Cytochrome P450 2E1/CYP2E1 recombinant protein (Position: H355-S493).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M00672-2 is reactive to CYP2E1 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
56 kDa
Calculated molecular weight
56.849kDa
Background of Cytochrome P450 2E1
Cytochrome P450 2E1 (abbreviated CYP2E1), a member of the cytochrome P450 mixed-function oxidase system, is involved in the metabolism of xenobiotics in the body. In humans, the CYP2E1 enzyme is encoded by the CYP2E1 gene. It is mapped to 10q26.3. While it is involved in the oxidative metabolism of a small range of substrates (mostly small polar molecules), there are many important drug interactions mediated by CYP2E1. Most drugs undergo deactivation by CYP2E1, either directly or by facilitated excretion from the body. Also, many substances are bioactivated by CYP2E1 to form their active compounds. In addition, CYP2E1 is an important enzyme for the conversion of ethanol to acetaldehyde and to acetate in humans. In the conversion sequence of acetyl-CoA to glucose, CYP2E1 transforms acetone via acetol into propylene glycol and methylglyoxal, the precursors of pyruvate, acetate and lactate.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00672-2 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Positive Control
WB: human HCCP tissue, rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cytochrome P450 2E1/CYP2E1 using anti-Cytochrome P450 2E1/CYP2E1 antibody (M00672-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HCCP tissue lysates,
Lane 2: rat liver tissue lysates,
Lane 3: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytochrome P450 2E1/CYP2E1 antigen affinity purified monoclonal antibody (Catalog # M00672-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for Cytochrome P450 2E1/CYP2E1 at approximately 56 kDa. The expected band size for Cytochrome P450 2E1/CYP2E1 is at 56 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CYP2E1 (Source: Uniprot.org, NCBI)
Gene Name
CYP2E1
Full Name
Cytochrome P450 2E1
Weight
56.849kDa
Superfamily
cytochrome P450 family
Alternative Names
Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; RNA helicase p68; DDX5; G17P1, HELR, HLR1 CYP2E1 CPE1, CYP2E, P450-J, P450C2E cytochrome P450 family 2 subfamily E member 1 cytochrome P450 2E1|4-nitrophenol 2-hydroxylase|CYPIIE1|cytochrome P450, family 2, subfamily E, polypeptide 1|cytochrome P450, subfamily IIE (ethanol-inducible), polypeptide 1|cytochrome P450-J|flavoprotein-linked monooxygenase|microsomal monooxygenase|xenobiotic monooxygenase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP2E1, check out the CYP2E1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP2E1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cytochrome P450 2E1/CYP2E1 Antibody Picoband® (monoclonal, 2C7G1) (M00672-2)
Hello CJ!
M00672-2 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of ammonium pyrrolidine dithiocarbamate (PDTC) on NF-κB activation and CYP2E1 content of rats with immunological liver injury
Protective effect of wild Corni fructus methanolic extract against acute alcoholic liver injury in mice
Furocoumarins affect hepatic cytochrome P450 and renal organic ion transporters in mice
An improved in vitro method for screening toxin and medicine targeting CYP2E1
Schisandra sphenanthera extract (Wuzhi Tablet) protects against chronic-binge and acute alcohol-induced liver injury by regulating the NRF2-ARE pathway in mice
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