Product Info Summary
SKU: | A06539 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Cysteine protease ATG4A ATG4A Antibody
SKU/Catalog Number
A06539
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Cysteine protease ATG4A ATG4A Antibody (Catalog # A06539). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG4A antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Cysteine protease ATG4A ATG4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06539)
Host
Rabbit
Contents
ATG4A antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A. The immunogen is located within the last 50 amino acids of ATG4A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ATG4A antibody is human, mouse and rat reactive. At least two isoforms of ATG4A are known to exist; this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins.
Reactive Species
A06539 is reactive to ATG4A in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
45378 MW
Background of ATG4A
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4A, also known as AUTL2, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06539 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG4A antibody can be used for detection of ATG4A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of ATG4A in EL4 cell lysate with ATG4A antibody at (A) 1 and (B) 2 μg/ml.
Click image to see more details
Immunohistochemistry of ATG4A in human spleen tissue with ATG4A antibody at 5 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For ATG4A (Source: Uniprot.org, NCBI)
Gene Name
ATG4A
Full Name
Cysteine protease ATG4A
Weight
45378 MW
Superfamily
peptidase C54 family
Alternative Names
Autophagy related 4A cysteine peptidase, APG4-A, APG4-A, AUTL2 ATG4A APG4A, AUTL2 autophagy related 4A cysteine peptidase cysteine protease ATG4A|APG4 autophagy 4 homolog A|ATG4 autophagy related 4 homolog A|AUT-like 2, cysteine endopeptidase|autophagin 2|autophagy-related cysteine endopeptidase 2|autophagy-related protein 4 homolog A|hAPG4A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG4A, check out the ATG4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cysteine protease ATG4A ATG4A Antibody (A06539)
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