Anti-BRAF Rabbit Monoclonal Antibody, Clone#RM308

Product Name

Anti-BRAF Rabbit Monoclonal Antibody, Clone#RM308

View all B-Raf Antibodies

SKU/Catalog Number

M00075-2

Size

100uL

Form

Liquid

Description

Boster Bio Anti-BRAF Rabbit Monoclonal Antibody, Clone#RM308 (Catalog # M00075-2). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BRAF Rabbit Monoclonal Antibody, Clone#RM308 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00075-2)

Host

Rabbit

Contents

50% Glycerol/PBS with 1% BSA and 0.09% sodium azide

Clonality

Monoclonal

Clone Number

RM308

Isotype

Rabbit IgG

Immunogen

A peptide corresponding to the residues near N-terminus of human B-raf

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

This antibody reacts to human serine/threonine-protein kinase B-raf (BRAF). It may also react to mouse B-raf, as predicted by immunogen homology.

Reactive Species

M00075-2 is reactive to BRAF in Human

Applications

M00075-2 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

84437 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (IHC): 1:200-1:500 dilution
WB: 1:100-1:200 dilution.

Validation Images & Assay Conditions

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Figure 1. Western Blotting result
Western Blot of K562 cells lysate using anti-B-raf rabbit monoclonal antibody (Clone RM308) at a 1:100 dilution.

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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-B-raf rabbit monoclonal antibody (Clone RM308) at a 1:500 dilution.

Gene/Protein Information For BRAF (Source: Uniprot.org, NCBI)

Alternative Names

94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); BRaf; B-Raf; BRAF1; B-RAF1; BRAF1MGC126806; EC 2.7.11.1; MGC138284; murine sarcoma viral (v-raf) oncogene homolog B1; NS7; p94; Proto-oncogene B-Raf; RAFB1; serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B1FLJ95109 BRAF B-RAF1, B-raf1, NS7, RAFB1, BRAF B-Raf proto-oncogene, serine/threonine kinase serine/threonine-protein kinase B-raf|94 kDa B-raf protein|B-Raf proto-oncogene serine/threonine-protein kinase (p94)|B-Raf serine/threonine-protein|murine sarcoma viral (v-raf) oncogene homolog B1|proto-oncogene B-Raf|v-raf murine sarcoma viral oncogene homolog B|v-raf murine sarcoma viral oncogene homolog B1

For more info on BRAF, check out the BRAF Infographic

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In this infographic, you will see the following information for BRAF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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