Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody

CYP2R1 antibody

Boster Bio Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody catalog # A03557. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey, Mouse.

Product Info Summary

SKU: A03557
Size: 100μl
Reactive Species: Human, Monkey, Mouse
Host: Rabbit
Application: ELISA, IHC-P, WB

Product Name

Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody

View all CYP2R1 Antibodies

SKU/Catalog Number

A03557

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody catalog # A03557. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Monkey, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03557)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

The antiserum was produced against synthesized peptide derived from human CYP2R1. AA range:251-300

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A03557 is reactive to CYP2R1 in Human, Monkey, Mouse

Reconstitution

Observed Molecular Weight

39 kDa

Calculated molecular weight

57359 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A03557 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000

Validation Images & Assay Conditions

Gene/Protein Information For CYP2R1 (Source: Uniprot.org, NCBI)

Gene Name

CYP2R1

Full Name

Vitamin D 25-hydroxylase

Weight

57359 MW

Superfamily

cytochrome P450 family

Alternative Names

Vitamin D 25-hydroxylase;1.14.14.24 ;Cytochrome P450 2R1;CYP2R1; CYP2R1 cytochrome P450 family 2 subfamily R member 1 vitamin D 25-hydroxylase|cytochrome P450 2R1|cytochrome P450, family 2, R1|cytochrome P450, family 2, subfamily R, polypeptide 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CYP2R1, check out the CYP2R1 Infographic

CYP2R1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CYP2R1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Vitamin D 25-hydroxylase CYP2R1 Antibody

Question

We are currently using anti-CYP2R1 antibody A03557 for monkey tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, monkey, mouse. Is it likely that the antibody can work on feline tissues as well?

G. Krishna

Verified customer

Asked: 2020-02-11

Answer

The anti-CYP2R1 antibody (A03557) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-11

Question

Can you help my question with product A03557, anti-CYP2R1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-12-26

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A03557 anti-CYP2R1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-12-26

Question

Is this A03557 anti-CYP2R1 antibody reactive to the isotypes of CYP2R1?

Verified Customer

Verified customer

Asked: 2019-09-03

Answer

The immunogen of A03557 anti-CYP2R1 antibody is Synthesized peptide derived from the Internal region of human CYP2R1. at AA rangle: 220-300. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-09-03

Question

Here is the WB image, lot number and protocol we used for sperm using anti-CYP2R1 antibody A03557. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-05

Question

Is a blocking peptide available for product anti-CYP2R1 antibody (A03557)?

E. Kulkarni

Verified customer

Asked: 2017-02-15

Answer

We do provide the blocking peptide for product anti-CYP2R1 antibody (A03557). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-02-15

Question

Will anti-CYP2R1 antibody A03557 work for WB with sperm?

W. Jones

Verified customer

Asked: 2016-02-19

Answer

According to the expression profile of sperm, CYP2R1 is highly expressed in sperm. So, it is likely that anti-CYP2R1 antibody A03557 will work for WB with sperm.

Boster Scientific Support

Answered: 2016-02-19

Order DetailsPrice
A03557

100uL

$399

Free Secondary Antibody

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A03557
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$399.00

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