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Product Info Summary
| SKU: | A02121 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-CYP27A1 Antibody
SKU/Catalog Number
A02121
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CYP27A1 Antibody catalog # A02121. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYP27A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02121)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant protein corresponding to human CYP27A1 300-500aa.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02121 is reactive to CYP27A1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
60235 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02121 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of CYP27A1 using Anti-CYP27A1 Antibody (A02121).
ICC staining CYP27A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-CYP27A1 Antibody A02121
Western blot analysis of CYP27A1 on different tissue lysates using anti-CYP27A1 antibody at 1/500 dilution.Positive control:
Lane 1: Rat heart
Lane 2: Human liver
Lane 2: Human kidney
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CYP27A1 (Source: Uniprot.org, NCBI)
Gene Name
CYP27A1
Full Name
Sterol 26-hydroxylase, mitochondrial
Weight
60235 MW
Superfamily
cytochrome P450 family
Alternative Names
Sterol 26-hydroxylase, mitochondrial;1.14.15.15 CYP27A1 CP27, CTX, CYP27 cytochrome P450 family 27 subfamily A member 1 sterol 26-hydroxylase, mitochondrial|5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase|5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase|cholestanetriol 26-monooxygenase|cytochrome P-450C27/25|cytochrome P450 27|cytochrome P450, family 27, subfamily A, polypeptide 1|cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous xanthomatosis), polypeptide 1|sterol 27-hydroxylase|vitamin D(3) 25-hydroxylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYP27A1, check out the CYP27A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYP27A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYP27A1 Antibody (A02121)
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No publications found for A02121
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