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Product Info Summary
| SKU: | PB9103 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Cyclin D3/CCND3 Antibody Picoband®
SKU/Catalog Number
PB9103
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cyclin D3/CCND3 Antibody Picoband® catalog # PB9103. Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin D3/CCND3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9103)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cyclin D3 recombinant protein (Position: Q136-L292). Human Cyclin D3 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat Cyclin D3, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9103 is reactive to CCND3 in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
33 kDa
Calculated molecular weight
32520 MW
Background of Cyclin D3
CCND3, also called Cyclin D3, is a protein that in humans is encoded by the CCND3 gene. It is mapped to 6p21.1. The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. In addition, overexpression of CCND3 upregulated the translational activity in HeLa cells in a dose-dependent manner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9103 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat
Positive Control
WB: rat testis tissue, rat thymus tissue, rat lung tissue, rat ovary tissue, JURKAT whole cell, A549 whole cell, MCF-7 whole cell, HELA whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cyclin D3 using anti-Cyclin D3 antibody (PB9103).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: recombinant human Cyclin D3 protein 0.5ng.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D3 antigen affinity purified polyclonal antibody (Catalog # PB9103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D3 at approximately 25KD. The expected band size for Cyclin D3 is at 25KD.
Click image to see more details
Figure 2. Western blot analysis of Cyclin D4 using anti-Cyclin D4 antibody (PB9103).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
lane 1: rat testis tissue lysate,
lane 2: rat thymus tissue lysate,
lane 3: rat lung tissue lysate,
lane 4: rat ovary tissue lysate,
lane 5: JURKAT whole cell lysate,
lane 6: A549 whole cell lysate,
lane 7: MCF-7 whole cell lysate,
lane 8: HELA whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin D4 antigen affinity purified polyclonal antibody (Catalog # PB9103) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin D4 at approximately 33KD. The expected band size for Cyclin D4 is at 33KD.
Protein Target Info & Infographic
Gene/Protein Information For CCND3 (Source: Uniprot.org, NCBI)
Gene Name
CCND3
Full Name
G1/S-specific cyclin-D3
Weight
32520 MW
Superfamily
cyclin family
Alternative Names
G1/S-specific cyclin-D3;CCND3; CCND3 cyclin D3 G1/S-specific cyclin-D3|D3-type cyclin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCND3, check out the CCND3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCND3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin D3/CCND3 Antibody Picoband® (PB9103)
Hello CJ!
PB9103 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Co-culture with TM4 cells enhances the proliferation and migration of rat adipose-derived mesenchymal stem cells with high stemness
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Customer Q&As
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7 Customer Q&As for Anti-Cyclin D3/CCND3 Antibody Picoband®
Question
Is a blocking peptide available for product anti-Cyclin D3/CCND3 antibody (PB9103)?
Verified Customer
Verified customer
Asked: 2020-04-10
Answer
We do provide the blocking peptide for product anti-Cyclin D3/CCND3 antibody (PB9103). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-10
Question
We are currently using anti-Cyclin D3/CCND3 antibody PB9103 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The anti-Cyclin D3/CCND3 antibody (PB9103) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-11
Question
I have attached the WB image, lot number and protocol we used for thymus umbilical cord blood using anti-Cyclin D3/CCND3 antibody PB9103. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-29
Question
I am looking for to test anti-Cyclin D3/CCND3 antibody PB9103 on human thymus umbilical cord blood for research purposes, then I may be interested in using anti-Cyclin D3/CCND3 antibody PB9103 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-18
Answer
The products we sell, including anti-Cyclin D3/CCND3 antibody PB9103, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-18
Question
Will anti-Cyclin D3/CCND3 antibody PB9103 work on horse Flow Cytometry with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
Our lab technicians have not validated anti-Cyclin D3/CCND3 antibody PB9103 on horse. You can run a BLAST between horse and the immunogen sequence of anti-Cyclin D3/CCND3 antibody PB9103 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse cervix carcinoma in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-26
Question
Is this PB9103 anti-Cyclin D3/CCND3 antibody reactive to the isotypes of CCND3?
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
The immunogen of PB9103 anti-Cyclin D3/CCND3 antibody is E.coli-derived human Cyclin D3 recombinant protein (Position: Q136-L292). Human Cyclin D3 shares 94% and 93% amino acid (aa) sequences identity with mouse and rat Cyclin D3, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-28
Question
Would PB9103 anti-Cyclin D3/CCND3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-15
Answer
As indicated on the product datasheet, PB9103 anti-Cyclin D3/CCND3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-15
