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Product Info Summary
| SKU: | M00700 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00700
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody catalog # M00700. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00700)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HOD-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cyclin A1/A2
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00700 is reactive to CCNA2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
55 kDa
Calculated molecular weight
48551 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00700 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:100-1:500
IP 1:50
Positive Control
WB: human Hela whole cell, human Jurkat whole cell
IHC: human colon cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Cyclin A1/A2 using anti-Cyclin A1/A2 antibody (M00700).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cyclin A1/A2 antigen affinity purified monoclonal antibody (Catalog # M00700) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cyclin A1/A2 at approximately 55 kDa. The expected band size for Cyclin A1/A2 is at 49 kDa.
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Immunohistochemical analysis of paraffin-embedded human colon cancer, using Cyclin A1/A2 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For CCNA2 (Source: Uniprot.org, NCBI)
Gene Name
CCNA2
Full Name
Cyclin-A2
Weight
48551 MW
Superfamily
cyclin family
Alternative Names
Cyclin-A2;Cyclin-A;CCNA2;CCN1, CCNA; CCNA2 CCN1, CCNA cyclin A2 cyclin-A2|cyclin-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCNA2, check out the CCNA2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCNA2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody (M00700)
Hello CJ!
M00700 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wang S,Zhang C,Zhang X.Downregulation of long non‑coding RNA ANRIL promotes proliferation and migration in hypoxic human pulmonary artery smooth muscle cells.Mol Med Rep.2020 Feb;21(2):589-596.doi:10.3892/mmr.2019.10887.Epub 2019 Dec 17.PMID:31974617; PMC
Species: Human
M00700 usage in article: APP:WB, SAMPLE:HPASMCS, DILUTION:1:200
Wang C, Ge Q, Chen Z, Hu J, Li F, Song X, Xu H, Ye Z. Biomed Res Int. 2015;2015:304753. Doi: 10.1155/2015/304753. Epub 2015 Mar 30. A New Double Stranded Rna Suppresses Bladder Cancer Development By Upregulating P21 (Waf1/Cip1) Expression.
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