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Product Info Summary
| SKU: | M00328 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00328
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CYBB/Nox2 Rabbit Monoclonal Antibody catalog # M00328. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CYBB/Nox2 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00328)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GOH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CYBB
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00328 is reactive to CYBB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
47 kDa
Calculated molecular weight
65336 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00328 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:5K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of NOX2/CYBB/gp91phox expression in MCF-7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CYBB (Source: Uniprot.org, NCBI)
Gene Name
CYBB
Full Name
Cytochrome b-245 heavy chain
Weight
65336 MW
Alternative Names
Cytochrome b-245 heavy chain;1.-.-.-;CGD91-phox;Cytochrome b (558) subunit beta;Cytochrome b558 subunit beta;Heme-binding membrane glycoprotein gp91phox;NADPH oxidase 2;Neutrophil cytochrome b 91 kDa polypeptide;Superoxide-generating NADPH oxidase heavy chain subunit;gp91-1;gp91-phox;p22 phagocyte B-cytochrome;CYBB;NOX2; CYBB AMCBX2, CGD, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX cytochrome b-245 beta chain cytochrome b-245 heavy chain|CGD91-phox|NADPH oxidase 2|cytochrome b(558) subunit beta|cytochrome b-245 beta polypeptide|cytochrome b558 subunit beta|heme-binding membrane glycoprotein gp91phox|neutrophil cytochrome b 91 kDa polypeptide|p22 phagocyte B-cytochrome|superoxide-generating NADPH oxidase heavy chain subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CYBB, check out the CYBB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CYBB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CYBB/Nox2 Rabbit Monoclonal Antibody (M00328)
Hello CJ!
M00328 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou H,Wang H,Shi N,Wu F.Potential Protective Effects of the Water-Soluble Chinese Propolis on Hypertension Induced by High-Salt Intake. Clin Transl Sci.2020 Sep;13(5):907-915.doi:10.1111/cts.12770.Epub 2020 Apr 8.PMID:32112504.
Species: Rat
M00328 usage in article: APP:WB, SAMPLE:KIDNEY,HEART MUSCLE AND VASCULAR TISSUES, DILUTION:NA
Maternal inflammation activated ROS-p38 MAPK predisposes offspring to heart damages caused by isoproterenol via augmenting ROS generation
Graviola inhibits hypoxia-induced NADPH oxidase activity in prostate cancer cells reducing their proliferation and clonogenicity
Du Z, Yang Q, Zhou T, Liu L, Li S, Chen S, Gao C. Mol Med Rep. 2014 Dec;10(6):2861-7. Doi: 10.3892/Mmr.2014.2653. Epub 2014 Oct 15. D?
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CYBB/Nox2 Rabbit Monoclonal Antibody
Question
Would anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 work on pig WB with liver?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
Our lab technicians have not tested anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-23
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-27
Question
Is there a BSA free version of anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 available?
Verified Customer
Verified customer
Asked: 2019-01-23
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-23
Question
Would anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 work for WB with liver?
S. Roberts
Verified customer
Asked: 2018-08-31
Answer
According to the expression profile of liver, CYBB is highly expressed in liver. So, it is likely that anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 will work for WB with liver.
Boster Scientific Support
Answered: 2018-08-31
Question
I see that the anti-CYBB/Nox2 Rabbit Monoclonal antibody M00328 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-15
Question
Is a blocking peptide available for product anti-CYBB/Nox2 Rabbit Monoclonal antibody (M00328)?
J. Carter
Verified customer
Asked: 2018-04-06
Answer
We do provide the blocking peptide for product anti-CYBB/Nox2 Rabbit Monoclonal antibody (M00328). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-06
