CYBB Antibodies

Cytochrome b-245 heavy chain (CYBB)

Critical part of the membrane-bound oxidase of phagocytes that generates superoxide. It's the terminal part of a respiratory chain that transports single electrons from cytoplasmic NADPH throughout the plasma membrane to molecular oxygen on the exterior.

Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.

Gene Information

Human
Gene Name:	CYBB
Uniprot:	P04839
Entrez:	1536

Family

Belongs to:
No superfamily

Alternative Names

CGD; CGD91-phox; Cytochrome b(558) subunit beta; cytochrome b-245 heavy chain; cytochrome b-245, beta polypeptide; Cytochrome b558 subunit beta; EC 1.6.3; GP91-1; GP91PHOX; GP91-PHOX; Heme-binding membrane glycoprotein gp91phox; NADPH oxidase 2; Neutrophil cytochrome b 91 kDa polypeptide; NOX2chronic granulomatous disease; p22 phagocyte B-cytochrome; p91-PHOX; Superoxide-generating NADPH oxidase heavy chain subunit

Molecular Weight

Mass (kDA):
65.336 kDA

Genomic Context

Human
Location:	Xp21.1-p11.4
Sequence:	X; NC_000023.11 (37780059..37813461)

Tissue Specificity

Detected in neutrophils (at protein level).

Subcellular Localizations

Cell membrane; Multi-pass membrane protein. As unassembled monomer may localize to the endoplasmic reticulum.

Diseases

• Granulomatous Disease, Chronic
• Granulomatous Disorder
• Inflammation
• Hypertensive Disease
• Infective Disorder
• Immunologic Deficiency Syndromes
• Ischemia
• Diabetes Mellitus
• Fibrosis
• Hypertrophy
• Atherosclerosis
• Hypoxia

• Tissue Adhesions
• Leukemia
• Malignant Neoplasms
• Inflammatory Response
• Cardiovascular Diseases
• Anoxia
• Infarction

Pathways

• Respiratory Burst
• Pathogenesis
• Cell Death
• Localization
• Inflammatory Response
• Phagocytosis
• Cell Proliferation
• Excretion
• Secretion
• Angiogenesis
• Transport
• Immune Response
• Vasoconstriction

• Aging
• Cell Adhesion
• Neuroprotection
• Glycosylation
• Chemotaxis
• Senescence
• Cytokine Production

PTMs

• Phosphorylation
• Oxidation
• Glycosylation
• Reduction
• Nitration
• Dephosphorylation
• Acetylation

• Nitrosylation
• Cleavage
• Prenylation
• Biotinylation
• Sumoylation
• Carboxylation
• Phosphorothioation

• Ribosylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CYBB

Boster Bio: Best Uses of the Cybb Marker

Are you confused about the CYBB Gene? This article will answer your questions regarding how this gene functions and its numerous uses. Keep reading to learn more about the CYBB gene and its protein. Boster Bio The best uses of Cybb Marker

CYBB gene

CYBB is the DNA methylation marker located on the genome of the human Ovaries. Boster's CYBB marker is designed to detect mutated genes in these Ovaries. Molecular biology techniques have a wide range of applications in the world of science. Scientists can manipulate DNA to create genetic material for a variety applications. Boster provides a full range of digital tools and reagents, including high-quality buffers that can reduce cross-linking intensity.

CYBB protein

The CYBB protein, also known as the cytochrome B protein, is a molecule found in many cells. It is a heterodimer comprising two polypeptides called p91-phox and p22-phox. It is also called 608508 or Phox. Many different biological functions are carried out by the cytochrome B protein.

The CYBB protein has been employed in a range of research applications. It is a crucial biomarker for many fields, including cancer neurodevelopmental biology, inflammation, and neurosciences. Boster Bio antibodies have been tested against 250 different tissues to confirm their specificity and affinity. Boster ELISA kits are available through Tebu bio. If you're a scientist looking for an exact CYBB antibody, Boster Bio has you covered.

CYBB gene functions

The CYBB gene encodes a protein that contains 557 amino acids. Eissa and colleagues found that an intronic mutation known as a 978G-to-T transversion within intron 5 resulted in a variety of abnormally shaped mRNA products in a study. The mother of the child who was diagnosed with CGD was a patient for a long time of chronic skin infections as well as autosomal recessive CGD. The cytokeratins gene (CybB) protein was also identified in her blood. This resulted in an NBT test which was positive for a CYBB gene mutation. The results of dihydrorhodamine flow cytometry indicated that the mother was a carrier of X-linked CGD.

There have been connections between the CYBB gene and MS. The polymorphisms within this gene were associated with the development of MS and systemic Lupus Erymatosus and Sjogren's syndrome. It was not known whether the CYBB gene was responsible for the disease or not. It is not yet clear whether the CYBB genes play some role in MS pathogenesis. The NOX2 gene plays a significant part of the disease-suppressor mechanism.

Real-time RTPCR was used to detect CYBB gene expression in an investigation of Italian MS patients. The CYBB gene expression in 21 cases of RRMS was measured in PBMCs. The study was conducted with only females. The patients were found to have an 1.43-fold significant increase in the CYBB gene in comparison to controls. These results were consistent with the high CD4+ cell percentage within the heterogeneous pool of PBMCs.

A variety of biological tests can detect CYBB by using antibodies. Biological assays that use CYBB antibodies typically employ mouse and rabbit blood samples. In many cases it is the case that the CYBB gene is expressed in an splicing pattern that is heterogeneous. Boster Bio developed antibodies against the CYBB gene from mouse and rabbit tissues. The heavy chain of the Cytochrome B-245 gene is involved in the production of superoxide. It is also a part of the respiratory chain. It also regulates the pH of cells.

CYBB gene uses

The CYBB gene plays an important role in the progression of chronic granulomatous illness. The CYBB gene was first identified in this condition by Orkin in 2007. The paper was published in Nature within four days, and was a significant improvement in molecular genetics. It was one of the first attempts to utilize positional cloning to identify the gene that causes disease without knowing the protein encoded by it.

The SpCas9/sg/ODN genome-editing system retains its high degree of specificity. Both ODN donor and Cas9/sg are able to cause genomic deletions. Circularization identifies the ON- and OT sites. The CHANGE-seq method provides high-throughput analysis. The sequencing displays the number of reads at the respective sites. In the case of the CYBB gene, there are approximately two million reads.

The CYBB gene is employed in the ex vivo treatment of X-CGD. It is designed by using a chimeric promoter and is able to achieve 33% per cell DHR. The gene can be introduced into phagocyte cells by being specifically engineered. The CYBB MRNA that is released by AAV is also used to repair HSPCs that have X-CGD mutations.

Anti-NOX2-gp91phox/CYBB antibody is available in the market. You can purchase the antibody from many suppliers. Its catalog number is A00328-1. The CYBB antibody is a receptor for human mouse, rat, and human cell proteins. The peptide can also be employed in studies that require a certain length of the immunogen. If the anti-NOX2 antibody is not reacting with a specific immunegen, blocking peptide can be used to block the immunogen.