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Product Info Summary
| SKU: | M00031 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CXCR4 Monoclonal Antibody
SKU/Catalog Number
M00031
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CXCR4 Monoclonal Antibody catalog # M00031. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CXCR4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00031)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CID-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CXCR4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00031 is reactive to CXCR4 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
131 kDa
Calculated molecular weight
88068 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00031 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:100-1:200
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: human cervical carcinoma tissue
Validation Images & Assay Conditions
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Western blot analysis of CXCR4 expression in Jurkat cell lysate.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:500 dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human cervical carcinoma, using CXCR4 Antibody.
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Immunofluorescent analysis of Jurkat cells, using CXCR4 Antibody .
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CXCR4 (Source: Uniprot.org, NCBI)
Gene Name
CXCR4
Full Name
C-X-C chemokine receptor type 4
Weight
88068 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Signal transducer and activator of transcription 3;Acute-phase response factor;STAT3;APRF; CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS C-X-C motif chemokine receptor 4 C-X-C chemokine receptor type 4|CD184 |LPS-associated protein 3|SDF-1 receptor|chemokine (C-X-C motif) receptor 4|fusin|leukocyte-derived seven transmembrane domain receptor|lipopolysaccharide-associated protein 3|neuropeptide Y receptor Y3|neuropeptide Y3 receptor|seven transmembrane helix receptor|seven-transmembrane-segment receptor, spleen|stromal cell-derived factor 1 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR4, check out the CXCR4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR4 Monoclonal Antibody (M00031)
Hello CJ!
M00031 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gao W,Yang X,Du J,Wang H,Zhong H,Jiang J,Yang C.Glucocorticoid guides mobilization of bone marrow stem/progenitor cells via FPR and CXCR4 coupling.Stem Cell Res Ther.2021 Jan 7;12(1):16.doi:10.1186/s13287-020-02071-1.PMID:33413641;PMCID:PMC7791823.
Species: Mouse
M00031 usage in article: APP:IF, SAMPLE:MSCS AND EPCS, DILUTION:NA
Macrophage migration inhibitory factor%u2013CXCR4 is the dominant chemotactic axis in human mesenchymal stem cell recruitment to tumors
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Blockade of CXCL12/CXCR4 signaling inhibits intrahepatic cholangiocarcinoma progression and metastasis via inactivation of canonical Wnt pathway
Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CXCR4 Monoclonal Antibody
Question
Will anti-CXCR4 Monoclonal antibody M00031 work for IHC with fetal brain?
Verified Customer
Verified customer
Asked: 2020-02-07
Answer
According to the expression profile of fetal brain, CXCR4 is highly expressed in fetal brain. So, it is likely that anti-CXCR4 Monoclonal antibody M00031 will work for IHC with fetal brain.
Boster Scientific Support
Answered: 2020-02-07
Question
I have attached the WB image, lot number and protocol we used for fetal brain using anti-CXCR4 Monoclonal antibody M00031. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
Is this M00031 anti-CXCR4 Monoclonal antibody reactive to the isotypes of CXCR4?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
The immunogen of M00031 anti-CXCR4 Monoclonal antibody is A synthesized peptide derived from human CXCR4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-30
Question
Our lab were well pleased with the WB result of your anti-CXCR4 Monoclonal antibody. However we have seen positive staining in cervix carcinoma cell membrane using this antibody. Is that expected? Could you tell me where is CXCR4 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
From what I have seen in literature, cervix carcinoma does express CXCR4. Generally CXCR4 expresses in cell membrane. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799
Boster Scientific Support
Answered: 2019-07-03
Question
Would M00031 anti-CXCR4 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
As indicated on the product datasheet, M00031 anti-CXCR4 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-31
Question
We have observed staining in human peripheral blood leukocyte. What should we do? Is anti-CXCR4 Monoclonal antibody supposed to stain peripheral blood leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
Based on literature peripheral blood leukocyte does express CXCR4. Based on Uniprot.org, CXCR4 is expressed in oviduct epithelium, lung, fetal brain, fetal spleen, monocyte, peripheral blood leukocyte, peripheral blood lymphocyte, neutrophil, adrenal gland, colon, cervix carcinoma, among other tissues. Regarding which tissues have CXCR4 expression, here are a few articles citing expression in various tissues:
Adrenal gland, Pubmed ID: 14702039
Cervix carcinoma, Pubmed ID: 17081983, 18220336, 18669648, 20068231, 23186163
Colon, Pubmed ID: 15489334
Fetal brain, Pubmed ID: 8234909
Fetal spleen, Pubmed ID: 8325644
Lung, Pubmed ID: 8329116, 15815621
Monocyte, Pubmed ID: 8276799
Boster Scientific Support
Answered: 2019-05-21
Question
I see that the anti-CXCR4 Monoclonal antibody M00031 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-27
Question
I was wanting to use your anti-CXCR4 Monoclonal antibody for IHC for human fetal brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fetal brain identification?
Verified Customer
Verified customer
Asked: 2018-12-21
Answer
You can see on the product datasheet, M00031 anti-CXCR4 Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-12-21
Question
I have a question about product M00031, anti-CXCR4 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00031 anti-CXCR4 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-09-10
Question
Our lab want to know about to test anti-CXCR4 Monoclonal antibody M00031 on human fetal brain for research purposes, then I may be interested in using anti-CXCR4 Monoclonal antibody M00031 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
M. Krishna
Verified customer
Asked: 2018-08-29
Answer
The products we sell, including anti-CXCR4 Monoclonal antibody M00031, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-29
Question
Is a blocking peptide available for product anti-CXCR4 Monoclonal antibody (M00031)?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
We do provide the blocking peptide for product anti-CXCR4 Monoclonal antibody (M00031). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-27
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-CXCR4 Monoclonal antibody M00031. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-19
Question
Do you have a BSA free version of anti-CXCR4 Monoclonal antibody M00031 available?
Verified Customer
Verified customer
Asked: 2017-08-22
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CXCR4 Monoclonal antibody M00031 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-22
Question
We ordered your anti-CXCR4 Monoclonal antibody for WB on oviduct epithelium in the past. I am using mouse, and We are going to use the antibody for IHC next. My lab would like examining oviduct epithelium as well as neutrophil in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
K. Banerjee
Verified customer
Asked: 2016-12-05
Answer
I took a look at the website and datasheets of our anti-CXCR4 Monoclonal antibody and it seems that M00031 has been validated on mouse in both WB and IHC. Thus M00031 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-12-05
Question
Our lab want to know about using your anti-CXCR4 Monoclonal antibody for g protein-coupled receptor signaling pathway studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.
J. Evans
Verified customer
Asked: 2015-02-26
Answer
We appreciate your inquiry. This M00031 anti-CXCR4 Monoclonal antibody is tested on jurkat cells. It is guaranteed to work for IF, IHC, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-02-26
Question
We are currently using anti-CXCR4 Monoclonal antibody M00031 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on feline tissues as well?
N. Johnson
Verified customer
Asked: 2014-05-19
Answer
The anti-CXCR4 Monoclonal antibody (M00031) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-19
