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Product Info Summary
| SKU: | A00031-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CXCR4 Antibody
SKU/Catalog Number
A00031-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CXCR4 Antibody (Catalog # A00031-1). Tested in ELISA, WB, ICC, IF, Flow Cytometry, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
CXCR4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CXCR4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00031-1)
Host
Rabbit
Contents
CXCR4 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-CXCR4 antibody was raised against a peptide corresponding to 14 amino acids near the amino terminus of human CXCR4 isoform b. The immunogen is located within the first 50 amino acids of CXCR4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
CXCR4 Antibody is predicted to not cross-react with other CXCR familiy members.
Reactive Species
A00031-1 is reactive to CXCR4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
39746 MW
Background of CXCR4
CXCR4, a G-protein coupled receptor (GPCR) with seven transmembrane domains, is a CXC chemokine receptor specific for stromal-derived-factor-1 (SDF-1 or CXCL12). CXCR4 was initially discovered as one of the co-receptors for HIV entry into CD4+ T cells (1). Blocking CXCR4 could be potentially used as novel therapeutics for HIV treatment.
CXCR4 signaling plays an important role in the migration, proliferation and quiescence of hematopoietic stem cell and their retention within the bone marrow, where it has high levels of SDF-1/CXCL12(2). It has been demonstrated that CXCR4 signaling mediates CD20 up-regulation on B cells (3).
CXCR4 is highly expressed in more than 23 types of cancer, including breast cancer, ovarian cancer, melanoma, and prostate cancer, while there is very less or no expression of CXCR4 in healthy tissues. CXCR4 expression in cancer cells has been reported to be associated with tumor survival, growth and metastasis in tissues with high levels of SDF-1/CXCL12, such as lungs, liver and bone marrow (4,5).
CXCR4 has been shown to regulate neuronal migration, cell positioning and axon wiring (6,7). CXCR4 mutant mice displayed aberrant neuronal distribution, which implicates the role in neuronal disorders such as epilepsy. CXCR4 is also involved in WHIM syndrome (8). WHIM mutations in CXCR4 were recently found in patients with Waldenstrom's macroglobulinemia, and these mutations are correlated to clinical resistance to ibrutinib (9,10).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00031-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1 - 2 μg/mL; IP/ ICC: 2 μg/mL; IHC-P: 5 μg/mL; IF: 20 μg/mL; Flow Cyt: 0.1 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry, Immunocytochemistry and Immunofluorescence in human samples; Flow Cytometry in human and mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western Blot Validation of CXCR4 in HeLa Cells
Loading: 15 μg of lysates per lane. Antibodies: A00031-1 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines
Loading: 15 μg of lysates per lane. Antibodies: A00031-1 (1 μg/mL), (1 μg/mL), and beta-actin (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Validation with CXCR4 siRNA Knockdown in HeLa Cells
HeLa cells were transfected with control siRNAs (lane 1) or CXCR4 siRNAs (lane 2) Loading: 10 μg of HeLa whole cell lysates per lane. Antibodies: A00031-1 (2 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Animal Species Reactivity
Loading: Lysates/proteins at 20 μg per lane. Antibodies: A00031-1 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Recombinant Protein Test
Loading: CXCR4 partial recombinant protein. Lane 1: Anti-CXCR4 antibody (0.1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST. Lane 2: Coomassie blue staining. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
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Immunofluorescence Validation of CXCR4 in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling CXCR4 with A00031-1 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red). Image showing both membrane and cytoplasmic staining on HeLa cells.
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Flow Cytometry Validation of CXCR4 in HeLa Cells
Overlay histogram showing HeLa cells stained with A00031-1 (red line, 1μg/1x106 cells). 1 h incubation at 4˚C in 2% FBS/PBS. Followed by secondary antibody 488 goat anti-rabbit IgG (H+L) at 1/500 dilution for 1 h 4˚C.
Isotype control antibody (Green line) was mouse IgG1 (1μg/1x106 cells) used under the same conditions.
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Overexpression Validation of CXCR4 (Kozak et al., 2002)
U87MG and U87MG-CXCR4 extracts were included as negative and positive controls, respectively, for CXCR4 detection with anti-CXCR4 antibodies.
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WB Validation of CXCR4 in Human Metastatic Melanoma (Scala et al., 2006)
CXCR4 protein was detected in the human metastatic melanoma cell lines and human melanoma cell line (colo38), but not in the human primary melanocytes (MPR1) with anti-CXCR4 antibodies.
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Immunohistochemistry Validation of CXCR4 in Human Spleen
Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CXCR4 antibody (A00031-1) at 5 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A Goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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Immunocytochemistry Validation of CXCR4 in HeLa Cells
Immunocytochemical analysis of HeLa cells using anti-CXCR4 antibody (A00031-1) at 2 μg/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
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KO Validation of CXCR4 by Flow Cytometry (Ödemis, et al., 2010)
Astrocytes from wild-type or CXCR4 knockout mice were stained with primary antibodies against CXCR4 and FITC-labeled secondary antibodies, and subsequently subjected to flow cytometry. CXCR4−/− astrocytes (red) showed loss of CXCR4 cell-surface expression compared with wild-type cells (black).
Protein Target Info & Infographic
Gene/Protein Information For CXCR4 (Source: Uniprot.org, NCBI)
Gene Name
CXCR4
Full Name
C-X-C chemokine receptor type 4
Weight
39746 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
FB22, HM89, LAP3, LCR1, NPYR, WHIM, CD184, LESTR, NPY3R, NPYRL, HSY3RR, NPYY3R, D2S201E CXCR4 CD184, D2S201E, FB22, HM89, HSY3RR, LAP-3, LAP3, LCR1, LESTR, NPY3R, NPYR, NPYRL, NPYY3R, WHIM, WHIMS C-X-C motif chemokine receptor 4 C-X-C chemokine receptor type 4|CD184 |LPS-associated protein 3|SDF-1 receptor|chemokine (C-X-C motif) receptor 4|fusin|leukocyte-derived seven transmembrane domain receptor|lipopolysaccharide-associated protein 3|neuropeptide Y receptor Y3|neuropeptide Y3 receptor|seven transmembrane helix receptor|seven-transmembrane-segment receptor, spleen|stromal cell-derived factor 1 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CXCR4, check out the CXCR4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CXCR4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CXCR4 Antibody (A00031-1)
Hello CJ!
A00031-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Gao W,Yang X,Du J,Wang H,Zhong H,Jiang J,Yang C.Glucocorticoid guides mobilization of bone marrow stem/progenitor cells via FPR and CXCR4 coupling.Stem Cell Res Ther.2021 Jan 7;12(1):16.doi:10.1186/s13287-020-02071-1.PMID:33413641;PMCID:PMC7791823.
Species: Mouse
A00031-1 usage in article: APP:IF, SAMPLE:MSCS AND EPCS, DILUTION:NA
Chemokine CXCL12 and its receptor CXCR4 expression are associated with perineural invasion of prostate cancer
Tacrolimus promotes hepatocellular carcinoma and enhances CXCR4/SDF-1? expression?in vivo
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