Anti-Cullin 4a CUL4A Antibody

Product Name

Anti-Cullin 4a CUL4A Antibody

View all Cullin 4a Antibodies

SKU/Catalog Number

A01579

Size

100ul

Form

Liquid

Description

Boster Bio Anti-Cullin 4a CUL4A Antibody catalog # A01579. Tested in WB,ICC,IHC,Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cullin 4a CUL4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01579)

Host

Rabbit

Contents

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized non-phosphopeptide derived from human ADD1 around the phosphorylation site of serine 726 (T-P-S(p)-F-L).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01579 is reactive to CUL4A in Human

Applications

A01579 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

87680 MW

Background of Cullin 4a

Membrane-cytoskeleton-associated protein that promotes the assembly of the spectrin-actin network. Binds to calmodulin.

Yun Sok Lee, Mol. Cell. Biol., Jan 2007; 27: 438 - 452.
Kang Ho Kim, J. Biol. Chem., Dec 2004; 279: 51999 - 52006.
Marc Foretz, Mol. Cell. Biol., May 1999; 19: 3760 - 3768.
Jae Bum Kim, J. Clin. Invest., Jan 1998; 101: 1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:500-2,000
ICC: 1:50-1:200
IHC: 1:50-1:200
FC: 1:50-1:100

Validation Images & Assay Conditions

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Figure 2. Immunocytochemistry staining of CUL4A using Anti-Cullin 4a CUL4A Antibody (A01579).

ICC staining Cullin 4a in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde

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Figure 1. Western blotting validation for Anti-Cullin 4a CUL4A Antibody A01579

Western blot analysis of Cullin 4a on Hela cell lysates using anti-Cullin 4a antibody at 1/500 dilution.
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

Gene/Protein Information For CUL4A (Source: Uniprot.org, NCBI)

Alternative Names

CUL4A; CUL-4A; Cullin 4a; cullin-4A CUL4A cullin 4A cullin-4A|CUL-4A

For more info on CUL4A, check out the CUL4A Infographic

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In this infographic, you will see the following information for CUL4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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