Cullin-4A Antibodies

Cullin-4A (CUL4A)

Core part of numerous cullin-RING-based E3 ubiquitin-protein ligase complexes that mediate the ubiquitination of target proteins. As a scaffold protein can contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme.

The E3 ubiquitin-protein ligase activity of the complex is determined by the neddylation of the cullin subunit and is inhibited by the institution of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends upon the variable substrate recognition component.

Gene Information

Human
Gene Name:	CUL4A
Uniprot:	Q13619
Entrez:	8451

Family

Belongs to:
cullin family

Alternative Names

CUL4A; CUL-4A; Cullin 4a; cullin-4A

Molecular Weight

Mass (kDA):
87.68 kDA

Genomic Context

Human
Location:	13q34
Sequence:	13; NC_000013.11 (113208193..113267108)

Diseases

• Malignant Neoplasms
• Neoplasms
• Xeroderma Pigmentosum
• Xeroderma
• Immunologic Deficiency Syndromes
• Mammary Neoplasms
• Malignant Neoplasm Of Breast
• Hiv Infections
• Carcinoma
• Xeroderma Pigmentosum, Group E
• Carcinogenesis

• Cell Transformation, Neoplastic
• Malignant Neoplasm Of Liver
• Genomic Instability
• Hypoxia
• Malignant Paraganglionic Neoplasm
• Tumor Progression

Interacting Proteins

• CAND1
• DDB1
• DDB2
• RBBP5
• UBXN7

• VCP

Pathways

• Cell Cycle
• Proteolysis
• Dna Replication
• Dna Repair
• S Phase
• Cell Cycle Arrest
• Cell Proliferation
• Pathogenesis
• Mitosis
• Cell Growth
• Rna Interference
• Cell Division
• Response To Uv

• Methylation
• Dna Damage Checkpoint
• Cell Death
• Protein Ubiquitination
• Spermatogenesis
• V(d)j Recombination
• Reverse Transcription

PTMs

• Ubiquitination
• Phosphorylation
• Methylation

• Reduction
• Cleavage
• Dephosphorylation

Research Areas

• Apoptosis
• Cancer
• Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/CUL4A

Best Uses of the CUL4A Marker

The CUL4A Marker can be used to perform many biological assays. It can react with a variety of animal samples and can be monoclonal as well as polyclonal. Boster Bio has developed antibodies against Cullin-4A in rabbit and mouse. Catalysis is facilitated by Cullin-4A, a scaffold protein.

Cullin-4A

The Cullin-4A biomarker is a marker in human cells that detects DNA damage in cell culture. Its presence within the cell membrane is critical for tissue healing. It can be used for immunohistochemical staining, including for cancer research. This antibody detects the marker at a picogram-level level. The company sells a variety of immunological reagents, including immunohistochemical testing that is used in diagnostics and research.

The CUL4A gene encodes a single-copy protein, which is 87 kDa and belongs to the cullin family. This gene is frequently overexpressed in various types and types of cancers, including liver cancer. The expression of CUL4A has been found to be associated with the development and progression of various types of cancer. CUL4A is a key component of liver cancer. However, very little is known about its role. Researchers performed a series of experiments to investigate CUL4A expression in liver cancer and its association with various types of malignancy.

Its markers

A novel marker that promotes growth and migration in colorectal cancer cells has been discovered. This protein binds TP53 gene, which is a key player in cancer progression. Unfortunately, very little information exists about the correlation between TP53 and CUL4A expression patterns. Further research is needed to understand how this marker affects cancer cells, and the development of new treatments.

DDB2 and CSA were discovered to reveal the complex structure of CUL4A's protein. This discovery provides new insight into the molecular mechanisms that govern its action. This marker also links PTM to the expression of a particular protein. Further research is needed in order to determine if this protein is controlled by the cells' own inflammatory response. Further research is needed to understand the molecular mechanism behind these proteins' actions in order to develop a CUL4A drug.

CUL4A expression in advanced CRC patients is not associated with clinical outcomes. It is not known if CUL4A expression directly affects the survival rate of patients with CRC. CUL4A might be used to screen for early stages in the disease in cancer stem cells. CUL4A has been shown to be associated with several types of cancer. However, its expression in only one type tumor type is the best way to determine if this gene is present.

The CUL4A protein encodes a gene that regulates the cell cycle. It regulates the expressions of several CDK inhibitors that are vital for normal cell growth and survival. CUL4A can be overexpressed in cancer cells. This causes the cell cycle to be disrupted and leads to the uncontrolled proliferation malignant cells. Therefore, CUL4A is highly relevant for cancer research. It has many uses in science.

Its antibodies

The CUL4A gene is an important marker in the progression of cancer. Although it is not extensively studied in human liver cancer, some preliminary research suggests that CUL4A expression relates to patient survival, lymphatic and venous invasion, and HBeAg status. CUL4A knockdown was found to decrease motility and promote the proliferation of several human liver tumor cell lines. This suggests that CUL4A may be a promising target in the treatment of liver carcinoma.

Cullin-4A acts as a scaffold protein and contributes towards catalysis. Its high-affinity primary antibody has been used in biological assays since more than 25 years. To develop its CUL4A antibodies, Boster used rabbit and mouse. Its antibodies have been validated on multiple assays including Western Blotting and Immunohistochemistry. Researchers can monitor the progression or inhibit CUL4A with this antibody.

CUL4A expression increases cell migration and invasion in liver cancer cells. This protein is found mainly in liver carcinoma cells. These cells are predominantly mesenchymal, with very little epithelial. CUL4A is also known to increase cell viability and promote cell proliferation. It can also inhibit cell apoptosis. This protein likely promotes migration and invasion through other signaling pathways.

CUL4A is a single copy a gene that encodes cullin-like proteins with high expressions in the spleen. However, it is low expressed in the testis. It binds ring-box and DNA damage-binding proteins 1, forming the complex ubiquitin.ligaseE3. This mediates ubiquitination, and degradation of substrates. The CUL4A protein plays an important role in maintaining cellular physiology, and tumor development.

Its applications

Studies have shown that CUL4A expression deregulation or alteration can have a significant impact on cellular physiology. These studies suggest CUL4A in tumors may be a potential therapeutic target. CUL4A is not only a prognostic tool, but it also has many other potential uses, such as drug screening and the development personalized therapeutic strategies. Here is a review of some of these research.

In this study, 105 iCCA patients were taken from one institution. Tissue samples were fixed in formalin and then processed for immunohistochemistry. Immunohistochemical staining revealed a correlation between CUL4A protein and clinicopathologic features. Experiments on two stably CUL4-overexpressing iCCA cells revealed that CUL4 increases cell mobility potential.

CUL4A's encoder gene is located on the chromosome 13q34 and has 20 exons. The protein includes an 87-kDa subunit of cullin and is part a multifunctional ubiquitin–protein ligase E3 compound (CRL). It functions as a ubiquitin-conjugating enzyme, mediating ubiquitylation of substrates. CUL4A’s N terminal domain has an arc-shaped helical structure, which ensures that it binds a specific substrate adaptor or receptor.

The gene that encodes CUL4A has been implicated with non-small cell lung cancer (NSCLC). A recent study found that Cul4A expression correlates with the presence of ANXA10 protein in a variety of cancers. Patients without the gene had a lower chance of surviving (DFS) if their Cul4A levels were higher in lung cancer cells. The study included H460 and A549 lung cancer cells. A transfection vector containing the pcDNA3–Myc3–CUL4A gene was used to determine CUL4A's gene expression.