Product Info Summary
SKU: | A03475-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-CSNK2B Antibody Picoband™
View all Casein Kinase 2 beta Antibodies
SKU/Catalog Number
A03475-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CSNK2B Antibody Picoband™ catalog # A03475-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CSNK2B Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03475-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CSNK2B recombinant protein (Position: M1-R215).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03475-1 is reactive to CSNK2B in Human, Mouse, Rat
Applications
A03475-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
25 kDa
Calculated molecular weight
Background of Casein Kinase 2 beta
Casein kinase II subunit beta is a protein that in humans is encoded by the CSNK2B gene. This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a03475 1 csnk2b primary antibodies wb testing 1 a03475 1 csnk2b primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03475-1-csnk2b-primary-antibodies-wb-testing-1.jpg)
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Figure 1. Western blot analysis of CSNK2B using anti-CSNK2B antibody (A03475-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSNK2B antigen affinity purified polyclonal antibody (Catalog # A03475-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSNK2B at approximately 25 kDa. The expected band size for CSNK2B is at 25 kDa.
![a03475 1 csnk2b primary antibodies ihc testing 2 a03475 1 csnk2b primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1).
CSNK2B was detected in a paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03475 1 csnk2b primary antibodies ihc testing 3 a03475 1 csnk2b primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1).
CSNK2B was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03475 1 csnk2b primary antibodies ihc testing 4 a03475 1 csnk2b primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1).
CSNK2B was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a03475 1 csnk2b primary antibodies ihc testing 5 a03475 1 csnk2b primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a03475-1-csnk2b-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of CSNK2B using anti-CSNK2B antibody (A03475-1).
CSNK2B was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CSNK2B Antibody (A03475-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CSNK2B (Source: Uniprot.org, NCBI)
Gene Name
CSNK2B
Full Name
Casein kinase II subunit beta
Weight
Superfamily
casein kinase 2 subunit beta family
Alternative Names
Casein Kinase 2 beta; casein kinase 2, beta polypeptide; Casein kinase II beta subunit; casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSNK2B; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a CSNK2B CK2B, CK2N, CSK2B, Ckb1, Ckb2, G5A, POBINDS casein kinase 2 beta casein kinase II subunit beta|CK II beta|CSNK2B-LY6G5B-1072|CSNK2B-LY6G5B-1103|CSNK2B-LY6G5B-532|CSNK2B-LY6G5B-560|CSNK2B-LY6G5B-562|Casein kinase II beta subunit|alternative name: G5a, phosvitin|casein kinase 2, beta polypeptide|phosvitin|protein G5a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CSNK2B, check out the CSNK2B Infographic
![CSNK2B infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CSNK2B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CSNK2B Antibody Picoband™ (A03475-1)
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