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Product Info Summary
| SKU: | PB10060 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CSB/ERCC6 Antibody Picoband®
SKU/Catalog Number
PB10060
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CSB/ERCC6 Antibody Picoband® catalog # PB10060. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CSB/ERCC6 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10060)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CSB.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB10060 is reactive to ERCC6 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
170 kDa
Calculated molecular weight
168416 MW
Background of CSB
DNA excision repair protein ERCC-6 (also CS-B protein) is a protein that in humans is encoded by the ERCC6 gene. This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3, which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB10060 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: human Hela whole cell, human A431 whole cell, human Siha whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CSB using anti-CSB antibody (PB10060).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Siha whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CSB antigen affinity purified polyclonal antibody (Catalog # PB10060) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CSB at approximately 170 kDa. The expected band size for CSB is at 168 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ERCC6 (Source: Uniprot.org, NCBI)
Gene Name
ERCC6
Full Name
Chimeric ERCC6-PGBD3 protein
Weight
168416 MW
Alternative Names
DNA excision repair protein ERCC-6;3.6.4.-;ATP-dependent helicase ERCC6;Cockayne syndrome protein CSB;ERCC6;CSB; ERCC6 ARMD5, CKN2, COFS, COFS1, CSB, CSB-PGBD3, POF11, RAD26, UVSS1 ERCC excision repair 6, chromatin remodeling factor DNA excision repair protein ERCC-6|ERCC6-PGBD3 fusion protein|ATP-dependent helicase ERCC6|Chimeric CSB-PGBD3 protein|Chimeric ERCC6-PGBD3 protein|Cockayne syndrome group B protein|cockayne syndrome protein CSB|excision repair cross-complementation group 6|excision repair cross-complementing rodent repair deficiency, complementation group 6
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ERCC6, check out the ERCC6 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ERCC6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CSB/ERCC6 Antibody Picoband® (PB10060)
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No publications found for PB10060
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Customer Q&As
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1 Customer Q&As for Anti-CSB/ERCC6 Antibody Picoband®
Question
We are currently using anti-CSB/ERCC6 antibody PB10060 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, rat. Is it possible that the antibody can work on primate tissues as well?
D. Li
Verified customer
Asked: 2018-07-10
Answer
The anti-CSB/ERCC6 antibody (PB10060) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-10
