Product Info Summary
SKU: | M02100 |
---|---|
Size: | 100ul |
Reactive Species: | Human |
Host: | Mouse |
Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-Crk-L Monoclonal Antibody
SKU/Catalog Number
M02100
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Crk-L Monoclonal Antibody catalog # M02100. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Crk-L Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02100)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
5E12
Isotype
IgG
Immunogen
Purified recombinant fragment of human Crk-L expressed in E. Coli.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02100 is reactive to CRKL in Human
Reconstitution
Calculated molecular weight
33777 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02100 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Figure 3. ELISA validation of CRKL using Anti-Crk-L Monoclonal Antibody (M02100).
ELISA analysis of Crk-L antibody.Click image to see more details
Figure 2. Flow Cytometry validation of CRKL using Anti-Crk-L Monoclonal Antibody (M02100).
Flow cytometric (FCM) analysis of NIH/3T3 cells using Crk-L Monoclonal Antibody (blue) and negative control (red).Click image to see more details
Figure 1. Western blotting validation for Anti-Crk-L Monoclonal Antibody M02100
Western Blot (WB) analysis using Crk-L Monoclonal Antibody against human Crk-L (AA: 100-303) recombinant protein.Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For CRKL (Source: Uniprot.org, NCBI)
Gene Name
CRKL
Full Name
Crk-like protein
Weight
33777 MW
Superfamily
CRK family
Alternative Names
Crk-like protein;CRKL; CRKL CRK like proto-oncogene, adaptor protein crk-like protein|v-crk avian sarcoma virus CT10 oncogene homolog-like
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRKL, check out the CRKL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRKL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Crk-L Monoclonal Antibody (M02100)
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No publications found for M02100
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