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Product Info Summary
| SKU: | A00022-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-Bcr (Ab-177) Antibody
SKU/Catalog Number
A00022-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Bcr (Ab-177) Antibody (Catalog # A00022-1). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcr (Ab-177) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00022-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa. 175~179 (P-F-Y-V-N) derived from Human Bcr.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00022-1 is reactive to BCR in Human, Mouse
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
24656 MW
Background of BCR
GTPase-activating protein for RAC1 and CDC42. Promotes the exchange of RAC or CDC42-bound GDP by GTP, thereby activating them. Displays serine/threonine kinase activity.
Sattler M, et al. Mol Cell Biol. 1999 Nov; 19(11): 7473-7480.
Li S et al. EMBO J. 2001 Dec 3; 20(23): 6793-6804.
Million RP, et,al. Mol Cell Biol. 2004 Jun; 24(11): 4685-4695.
Malcolm A. Meyn, et al. . Biol. Chem., Oct 2006; 281: 30907
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00022-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 130 160 210kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BCR using anti-BCR antibody (A00022-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCR antigen affinity purified polyclonal antibody (Catalog # A00022-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for BCR.
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Immunofluorescence staining of methanol-fixed Hela cells using Bcr(Ab-177) Antibody #A00022-1.
Protein Target Info & Infographic
Gene/Protein Information For BCR (Source: Uniprot.org, NCBI)
Gene Name
BCR
Full Name
Breakpoint cluster region protein
Weight
24656 MW
Alternative Names
Cytotoxic T-lymphocyte protein 4;Cytotoxic T-lymphocyte-associated antigen 4;CTLA-4;CD152;CTLA4;CD152; BCR ALL1, CML, D22S11, D22S662, PHL, BCR BCR activator of RhoGEF and GTPase breakpoint cluster region protein|BCR, RhoGEF and GTPase activating protein|BCR/FGFR1 chimera protein|FGFR1/BCR chimera protein|breakpoint cluster region|renal carcinoma NY-REN-26
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCR, check out the BCR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcr (Ab-177) Antibody (A00022-1)
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No publications found for A00022-1
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Anti-Bcr (Ab-177) Antibody
Question
We have tried in the past anti-Bcr (Ab-177) antibody for IF on adenohypophysis a few years ago. I am using human, and We are going to use the antibody for WB next. We are interested in examining adenohypophysis as well as renal cell carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
I viewed the website and datasheets of our anti-Bcr (Ab-177) antibody and I see that A00022-1 has been validated on human in both IF and WB. Thus A00022-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-05-04
Question
Our team were happy with the WB result of your anti-Bcr (Ab-177) antibody. However we have observed positive staining in leukemic t-cell postsynapse, using this antibody. Is that expected? Could you tell me where is BCR supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
From literature, leukemic t-cell does express BCR. Generally BCR expresses in cell junction, synapse, postsynapse,. Regarding which tissues have BCR expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2020-02-26
Question
We are currently using anti-Bcr (Ab-177) antibody A00022-1 for mouse tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The anti-Bcr (Ab-177) antibody (A00022-1) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-28
Question
We have been able to see staining in human renal cell carcinoma. Are there any suggestions? Is anti-Bcr (Ab-177) antibody supposed to stain renal cell carcinoma positively?
Verified Customer
Verified customer
Asked: 2019-06-12
Answer
From what I have seen in literature renal cell carcinoma does express BCR. From what I have seen in Uniprot.org, BCR is expressed in adenohypophysis, brain, renal cell carcinoma, leukemic t-cell, cervix carcinoma, platelet, cervix carcinoma erythroleukemia, liver, among other tissues. Regarding which tissues have BCR expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Leukemic T-cell, Pubmed ID: 15144186, 19690332
Liver, Pubmed ID: 24275569
Platelet, Pubmed ID: 18088087
Boster Scientific Support
Answered: 2019-06-12
Question
Is this A00022-1 anti-Bcr (Ab-177) antibody reactive to the isotypes of BCR?
Verified Customer
Verified customer
Asked: 2019-02-07
Answer
The immunogen of A00022-1 anti-Bcr (Ab-177) antibody is Peptide sequence around aa. 175~179 (P-F-Y-V-N) derived from Human Bcr. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-07
Question
I am interested in to test anti-Bcr (Ab-177) antibody A00022-1 on mouse brain for research purposes, then I may be interested in using anti-Bcr (Ab-177) antibody A00022-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Jackson
Verified customer
Asked: 2018-06-14
Answer
The products we sell, including anti-Bcr (Ab-177) antibody A00022-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-06-14
Question
I was wanting to use your anti-Bcr (Ab-177) antibody for WB for mouse brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse brain identification?
H. Mangal
Verified customer
Asked: 2015-12-28
Answer
As indicated on the product datasheet, A00022-1 anti-Bcr (Ab-177) antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-28
Question
I would like using your anti-Bcr (Ab-177) antibody for inner ear morphogenesis studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
T. Kulkarni
Verified customer
Asked: 2013-03-13
Answer
Thank you for your inquiry. This A00022-1 anti-Bcr (Ab-177) antibody is tested on hela cells. It is guaranteed to work for IF, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-03-13
