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Product Info Summary
| SKU: | A03452-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Creatine Kinase MM/CKM Antibody Picoband®
View all Creatine Kinase, Muscle/CKMM Antibodies
SKU/Catalog Number
A03452-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Creatine Kinase MM/CKM Antibody Picoband® catalog # A03452-1. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Creatine Kinase MM/CKM Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03452-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Creatine Kinase MM recombinant protein (Position: F3-K381).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03452-1 is reactive to CKM in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
43 kDa
Calculated molecular weight
43.101kDa
Background of Creatine Kinase, Muscle/CKMM
Creatine kinase, muscle also known as CKM is a creatine kinase that in humans is encoded by the CKM gene. This gene is mapped to 19q13.32. The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03452-1 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human K562 whole cell, human CNE whole cell, human COLO-320 whole cell, rat heart tissue, rat lung tissue, mouse heart tissue, mouse lung tissue
IHC: rat cardiac muscle tissue, mouse cardiac muscle tissue, human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysate,
Lane 2: human CNE whole cell lysate,
Lane 3: human COLO-320 whole cell lysate,
Lane 4: rat heart tissue lysates,
Lane 5: rat lung tissue lysates,
Lane 6: mouse heart tissue lysates,
Lane 7: mouse lung tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Creatine Kinase MM antigen affinity purified polyclonal antibody (Catalog # A03452-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Creatine Kinase MM at approximately 43KD. The expected band size for Creatine Kinase MM is at 43KD.
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Figure 2. IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1).
Creatine Kinase MM was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1).
Creatine Kinase MM was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 4. IHC analysis of Creatine Kinase MM using anti-Creatine Kinase MM antibody (A03452-1).
Creatine Kinase MM was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Creatine Kinase MM Antibody (A03452-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CKM (Source: Uniprot.org, NCBI)
Gene Name
CKM
Full Name
Creatine kinase M-type
Weight
43.101kDa
Superfamily
ATP:guanido phosphotransferase family
Alternative Names
Creatine kinase M-type; Creatine kinase M chain; Creatine phosphokinase M-type ; CPK-M; M-CK; CKM; CKMM CKM CKMM, CPK-M, M-CK creatine kinase, M-type creatine kinase M-type|creatine kinase M chain|creatine kinase, muscle|creatine phosphokinase M-type
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CKM, check out the CKM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CKM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Creatine Kinase MM/CKM Antibody Picoband® (A03452-1)
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Customer Q&As
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3 Customer Q&As for Anti-Creatine Kinase MM/CKM Antibody Picoband®
Question
We are currently using anti-Creatine Kinase MM/CKM antibody A03452-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
The anti-Creatine Kinase MM/CKM antibody (A03452-1) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-02
Question
I would like to test anti-Creatine Kinase MM/CKM antibody A03452-1 on human vastus lateralis for research purposes, then I may be interested in using anti-Creatine Kinase MM/CKM antibody A03452-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
The products we sell, including anti-Creatine Kinase MM/CKM antibody A03452-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-19
Question
I was wanting to use your anti-Creatine Kinase MM/CKM antibody for ELISA for human vastus lateralis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human vastus lateralis identification?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
It shows on the product datasheet, A03452-1 anti-Creatine Kinase MM/CKM antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human vastus lateralis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-02
