This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
Facts about Creatine kinase M-type.
creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.
Human | |
---|---|
Gene Name: | CKM |
Uniprot: | P06732 |
Entrez: | 1158 |
Belongs to: |
---|
ATP:guanido phosphotransferase family |
CKM; CKMM; CKMMcreatine kinase M-type; Creatine kinase M chain; Creatine Kinase MM; creatine kinase, muscle; creatine kinase-M; EC 2.7.3; EC 2.7.3.2; M-CK
Mass (kDA):
43.101 kDA
Human | |
---|---|
Location: | 19q13.32 |
Sequence: | 19; NC_000019.10 (45306413..45322875, complement) |
Cytoplasm.
The CODEX(r), a multi-functional multiplex imaging system, can detect over 40 markers simultaneously. Its versatility allows multiplex imaging of multiple antigens, cytokines and metabolites. Learn about the history of BosterBio and his work. This article provides a brief biography for Steven Boster. It also contains a discussion on the CODEX(r), as well as a list if applications.
The CODEX(r), a new platform for multiplexed imaging, uses a panel-activator design that has been validated. It can multiplex 40 markers in one cycle. The system employs a unique three-step cleaning method that uses relative signal variance to clean each cycle. The process removes any high-level background objects and generates biaxial flow style lines of mutually exclusive marker lineage.
The CODEX is based on a multicycle process in which 56 antibodies were conjugated with unique DNA sequences. This multicycle imaging process generates a 58-dimensional image depicting protein expression for 56 antibodies and two nuclear markers. The image can then be rendered in seven different color options for further analysis. For example, CODEX imaging is capable of detecting cytokeratin, Ki-67, and cytokeratin expression in eight different CTCL tumor samples.
Multiplexed imaging is possible with over forty markers using the CODEX System. Three fluorescent oligonucleotides are used to stain samples. Each oligonucleotide corresponds to a distinct antibody. The reporter oligonucleotides then are removed from the sample. Repeat the CODEX cycle until all antibodies within a particular panel have been revealed. The data from each imaging session are then combined. This system allows you to simultaneously visualize and quantify up to 60 markers.
The CODEX(r), allows simultaneous imaging of more 40 markers. It is the first system worldwide to allow multiplexed image of more than 40 markers. CODEX imaging, unlike other methods, provides a high-quality image with small amounts protein. It is also very affordable and doesn't require expensive reagents.
The CODEX methodology is based on a well-designed antigen panel. It requires robust staining properties, and optimal imaging order. Multiplexed imaging of more than 40 markers can be achieved using FFPE cutaneous t cell lymphoma tissue. By using CODEX, researchers can assess the cellular types of the tumor in a variety of tissue microarrays.
The CODEX antibody panel comprises eight immunoregulatory proteins. CODEX antibody panels can simultaneously image all eight of these functional molecule on individual cells. This allows cancer researchers and scientists to develop effective immunotherapies. The CODEX immunopanel provides high-dimensional microenvironment (TME), along with assessment of tumor mutational load and gene expression. CODEX is also compatible with imaging using Imaging Mass Cytometry (IMC), or Tissue Based Cytometry, (TB-CIF).
The CODEX(r), System offers unprecedented image resolution and the ability to image the presence 40+ protein markers within one tissue section. The high-dimensional imaging platform enables detailed analysis of the tissue architecture. The CODEX(r), a multi-marker imaging system that allows multiplexed images of over 40 markers, allows researchers to explore complex diseases. So, why wait any longer? Start today with your next research assignment.
CODEX(r), a multiscale imaging platform, allows quantitative analysis of spatial associations in cells at multiple scales. This includes single cell resolution and pairwise proximity. This multiscale imaging platform makes it possible to study cell–cell interactions at unprecedented spatial resolution. CODEX offers a powerful workflow to multiplex image whole tissues. This advanced imaging platform allows researchers to better understand how biological processes are affected and how they respond to different spatial phenotypes.
CODEX implements an enhanced version of COCA. It also includes mechanisms for proactive secret-sharing and threshold cryptography. Multiplex Analysis Viewer(MAV) is a component of the CODEX Software Suite. MAV generates spatial coordinates each cell. The CODEX Software Suite also includes a general-purpose primitives toolkit that can be used for many purposes. MAV can visualize a variety of markers, including DAPI nuclear stained, CD8 and pancytokeratin.
Future Codex DNA instrumentation will include the SOLA-EDS technology. The CODEX(r),DNA platform provides fully automated benchtop solutions for synthetic biology. It also offers a catalog of synthetic genomes that includes SARS-CoV-2, monoclonal antibody treatments, small-molecule therapies, and diagnostic assays. In addition to this, CODEX DNA's synthetic genomes catalog also features a comprehensive list of gene-editing programs, including new vaccines for specific variants.
Steven Boster's past includes many historical landmarks. Despite his busy schedule, he always made time to spend with his family and friends. Boster, known by the nickname "he Who Converts Science in the Lavatory", was a family man. He loved to sing in his lower register in front of his family and friends. He also enjoyed sports and auto racing. He would often show to a friend’s car breakdown at 2 am, even though the temperature was below freezing. He treated friends and associates like family. His work ethic was unsurpassed.
Boster's extended family includes Natosha and Crystal Peck, as well three brothers. Steve is survived by his six grandchildren and his children, Donald, Sr. His siblings are Tammy Blanton and Kimberly Blanton as well as Jonathan Blanton and Cory Blanton. He was a Concordia Hall Staunton alumni. His death will leave a void in his family, but his legacy will continue to live on.
PMID: 3778496 by Perryman M.B., et al. Isolation and sequence analysis of a full-length cDNA for human M creatine kinase.
PMID: 2903158 by Trask R.V., et al. Developmental regulation and tissue-specific expression of the human muscle creatine kinase gene.