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Product Info Summary
| SKU: | M01320-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CPS1 Monoclonal Antibody
SKU/Catalog Number
M01320-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CPS1 Monoclonal Antibody catalog # M01320-1. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CPS1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01320-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFCG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CPS1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01320-1 is reactive to CPS1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
165 kDa
Calculated molecular weight
85957 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01320-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
Positive Control
WB: human Hela whole cell, human hepatocellular carcinoma tumor tissue (HCCT), human hepatocellular carcinoma paracancerous tissue (HCCP), rat liver tissue, mouse liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CPS1 using anti-CPS1 antibody (M01320-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human hepatocellular carcinoma tumor tissue (HCCT) lysates,
Lane 3: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 4: rat liver tissue lysates,
Lane 5: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPS1 antigen affinity purified monoclonal antibody (M01320-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CPS1 at approximately 165 kDa. The expected band size for CPS1 is at 165 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CPS1 (Source: Uniprot.org, NCBI)
Gene Name
CPS1
Full Name
Carbamoyl-phosphate synthase [ammonia], mitochondrial
Weight
85957 MW
Alternative Names
DNA-binding protein SATB1;Special AT-rich sequence-binding protein 1;SATB1; CPS1 CPSASE1, PHN carbamoyl-phosphate synthase 1 carbamoyl-phosphate synthase [ammonia], mitochondrial|carbamoyl-phosphate synthase (ammonia)|carbamoyl-phosphate synthase 1, mitochondrial|carbamoylphosphate synthetase I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CPS1, check out the CPS1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CPS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CPS1 Monoclonal Antibody (M01320-1)
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Customer Q&As
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2 Customer Q&As for Anti-CPS1 Monoclonal Antibody
Question
What is the sample target on Western blot analysis in your product image for M01320-1?
Verified customer
Asked: 2019-12-09
Answer
Western blot analysis of the Anti-CPS1 Monoclonal Antibody (M01320-1) expression in HeLa cell lysate.
Boster Scientific Support
Answered: 2019-12-10
Question
We are currently using anti-CPS1 Monoclonal antibody M01320-1 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?
A. Wu
Verified customer
Asked: 2013-02-14
Answer
The anti-CPS1 Monoclonal antibody (M01320-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-14
