Anti-CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Monoclonal Antibody

Product Name

Anti-CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Monoclonal Antibody

View all CPS1 Antibodies

SKU/Catalog Number

M01320

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Monoclonal Antibody (Catalog # M01320). Tested in IF, IHC applications. This antibody reacts with Human, Dog.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01320)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM615

Isotype

IgG1, kappa

Immunogen

Recombinant human CPS1 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01320 is reactive to CPS1 in Dog, Human

Applications

M01320 is guaranteed for IF, IHC Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

164939 MW

Background of CPS1

This monoclonal antibody recognizes a protein of 165kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC's occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Reconstitute with distilled water.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

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Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Anti-CPS1 Monoclonal Antibody (SPM615).

Gene/Protein Information For CPS1 (Source: Uniprot.org, NCBI)

Alternative Names

carbamoyl-phosphate synthase [ammonia], mitochondrial; carbamoyl-phosphate synthase 1, mitochondrial; carbamoyl-phosphate synthetase 1, mitochondrial; carbamoylphosphate synthetase I; Carbamoyl-phosphate synthetase I; CPSase I; CPSASE1; EC 6.3.4.16 CPS1 CPSASE1, PHN carbamoyl-phosphate synthase 1 carbamoyl-phosphate synthase [ammonia], mitochondrial|carbamoyl-phosphate synthase (ammonia)|carbamoyl-phosphate synthase 1, mitochondrial|carbamoylphosphate synthetase I

For more info on CPS1, check out the CPS1 Infographic

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In this infographic, you will see the following information for CPS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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