Anti-CP110/CCP110 Antibody Picoband®

CCP110 antibody

Boster Bio Anti-CP110/CCP110 Antibody Picoband® catalog # A05058-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Product Info Summary

SKU: A05058-1
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: ELISA, IF, IHC, ICC, WB

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Product Name

Anti-CP110/CCP110 Antibody Picoband®

View all CCP110 Antibodies

SKU/Catalog Number

A05058-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CP110/CCP110 Antibody Picoband® catalog # A05058-1. Tested in ELISA, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-CP110/CCP110 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05058-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human CP110 recombinant protein (Position: E51-H284).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A05058-1 is reactive to CCP110 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

110 kDa

Calculated molecular weight

39125 MW

Background of CCP110

Centriolar coiled-coil protein of 110 kDa also known as centrosomal protein of 110 kDa or CP110 is a protein that in humans is encoded by the CCP110 gene. This gene is mapped to chromosome 16p12.3. It is a cell cycle-dependent CDK substrate and regulates centrosome duplication. CP110 suppresses a cilia assembly program. CCP110 functions in a protein complex that participates in the transition of centrioles from basal body function to centrosomal function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05058-1 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Immunocytochemistry/Immunofluorescence, 5 μg/ml
ELISA, 0.1-0.5μg/ml

Positive Control

WB: human MOLT4 whole cell, human Hela whole cell, human PANC-1 whole cell, rat PC-12 whole cell, mouse RAW2647 whole cell
IHC: human breast cancer tissue, human colorectal adenocarcinoma tissue, human testicular germ cell tumor tissue
ICC/IF: HELA cell

Validation Images & Assay Conditions

Gene/Protein Information For CCP110 (Source: Uniprot.org, NCBI)

Gene Name

CCP110

Full Name

Centriolar coiled-coil protein of 110 kDa

Weight

39125 MW

Alternative Names

Centriolar coiled-coil protein of 110 kDa; Centrosomal protein of 110 kDa; CP110; Cep110; CCP110; CEP110; CP110; KIAA0419 CCP110 CP110, Cep110 centriolar coiled-coil protein 110 centriolar coiled-coil protein of 110 kDa|centriolar coiled-coil protein 110kDa|centrosomal protein CP110|centrosomal protein of 110 kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CCP110, check out the CCP110 Infographic

CCP110 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CCP110: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-CP110/CCP110 Antibody Picoband®

Question

I see that the anti-CP110/CCP110 antibody A05058-1 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-03-05

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-03-05

Question

Is this A05058-1 anti-CP110/CCP110 antibody reactive to the isotypes of CCP110?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

The immunogen of A05058-1 anti-CP110/CCP110 antibody is E. coli-derived human CP110 recombinant protein (Position: E51-H284). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-25

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lymphoblast using anti-CP110/CCP110 antibody A05058-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-24

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-24

Question

Is a blocking peptide available for product anti-CP110/CCP110 antibody (A05058-1)?

Verified Customer

Verified customer

Asked: 2019-11-22

Answer

We do provide the blocking peptide for product anti-CP110/CCP110 antibody (A05058-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-11-22

Question

I was wanting to use your anti-CP110/CCP110 antibody for IHC for mouse lymphoblast on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse lymphoblast identification?

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

It shows on the product datasheet, A05058-1 anti-CP110/CCP110 antibody has been tested for ELISA, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lymphoblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-25

Question

We are currently using anti-CP110/CCP110 antibody A05058-1 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on monkey tissues as well?

R. Huang

Verified customer

Asked: 2018-11-30

Answer

The anti-CP110/CCP110 antibody (A05058-1) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-30

Question

Does anti-CP110/CCP110 antibody A05058-1 work for IHC with lymphoblast?

Verified Customer

Verified customer

Asked: 2018-09-07

Answer

According to the expression profile of lymphoblast, CCP110 is highly expressed in lymphoblast. So, it is likely that anti-CP110/CCP110 antibody A05058-1 will work for IHC with lymphoblast.

Boster Scientific Support

Answered: 2018-09-07

Question

Is there a BSA free version of anti-CP110/CCP110 antibody A05058-1 available?

Verified Customer

Verified customer

Asked: 2017-08-04

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CP110/CCP110 antibody A05058-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-08-04

Question

My question regarding product A05058-1, anti-CP110/CCP110 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Zhang

Verified customer

Asked: 2015-04-03

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A05058-1 anti-CP110/CCP110 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2015-04-03

Question

We are interested in to test anti-CP110/CCP110 antibody A05058-1 on mouse lymphoblast for research purposes, then I may be interested in using anti-CP110/CCP110 antibody A05058-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Anderson

Verified customer

Asked: 2013-03-25

Answer

The products we sell, including anti-CP110/CCP110 antibody A05058-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-03-25

Question

Would anti-CP110/CCP110 antibody A05058-1 work on pig WB with brain?

D. Wu

Verified customer

Asked: 2013-02-05

Answer

Our lab technicians have not tested anti-CP110/CCP110 antibody A05058-1 on pig. You can run a BLAST between pig and the immunogen sequence of anti-CP110/CCP110 antibody A05058-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig brain in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-02-05

Question

Here is the WB image, lot number and protocol we used for lymphoblast using anti-CP110/CCP110 antibody A05058-1. Please let me know if you require anything else.

A. Yang

Verified customer

Asked: 2013-01-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-01-10

Question

Does A05058-1 anti-CP110/CCP110 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

N. Li

Verified customer

Asked: 2013-01-02

Answer

It shows on the product datasheet, A05058-1 anti-CP110/CCP110 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-01-02

Order DetailsPrice
A05058-1

100μg

$370
A05058-1-10ug

10μg sample (liquid)

$99
A05058-1-Biotin

100 μg Biotin conjugated

$570
A05058-1-Cy3

100 μg Cy3 conjugated

$570
A05058-1-Dylight488

100 μg Dylight488 conjugated

$570
A05058-1-Dylight550

100 μg Dylight550 conjugated

$570
A05058-1-Dylight594

100 μg Dylight594 conjugated

$570
A05058-1-FITC

100 μg FITC conjugated

$570
A05058-1-HRP

100 μg HRP conjugated

$570
A05058-1-APC

100 μg APC conjugated

$670
A05058-1-PE

100 μg PE conjugated

$670
A05058-1-iFluor647

100 μg iFluor647 conjugated

$670
A05058-1-carrier-free

Carrier Free

$370

More conjugates/formats

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A05058-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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