Anti-Connexin 40/GJA5 Antibody Picoband®

Product Name

Anti-Connexin 40/GJA5 Antibody Picoband®

View all Connexin 40/GJA5 Antibodies

SKU/Catalog Number

PA1368

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Connexin 40/GJA5 Antibody catalog # PA1368. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Connexin 40/GJA5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1368)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Connexin 40/GJA5, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1368 is reactive to GJA5 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

50 kDa

Calculated molecular weight

40380 MW

Background of Connexin 40/GJA5

Connexin proteins oligomerize to form intercellular channels, called gap junctions, through which ions and small molecules move between adjacent cells. Connexin 40 (Gap junction alpha-5 protein) is a protein that in humans is encoded by the GJA5 gene.This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1368 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Human, Rat

Positive Control

WB: Mouse Heart Tissue, Mouse Heart Tissue
IHC: Rat Cardiac Muscle Tissue

Validation Images & Assay Conditions

Gene/Protein Information For GJA5 (Source: Uniprot.org, NCBI)

Alternative Names

Gap junction alpha-5 protein;Connexin-40;Cx40;GJA5; GJA5 ATFB11, CX40 gap junction protein alpha 5 gap junction alpha-5 protein|connexin 40|gap junction protein alpha 5 40kDa

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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