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Product Info Summary
| SKU: | M01050 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC |
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Product info
Product Name
Anti-Connexin 32 (Gap Junction Protein) Monoclonal Antibody
View all Connexin 32/GJB1 Antibodies
SKU/Catalog Number
M01050
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Connexin 32 (Gap Junction Protein) Monoclonal Antibody (Catalog # M01050). Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Connexin 32 (Gap Junction Protein) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01050)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: GJB1/1753
Isotype
IgG
Immunogen
Recombinant human GJB1 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M01050 is reactive to GJB1 in Human, Mouse, Rat
Reconstitution
Reconstitute with distilled water.
Observed Molecular Weight
68 kDa
Calculated molecular weight
32025 MW
Background of Connexin 32/GJB1
This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called 'connexons' that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane therefore, one connexin usually cannot fully substitute for another.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01050 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Liver stained with Anti-Connexin 32 Monoclonal Antibody (GJB1/1753)
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-Connexin 32 Monoclonal Antibody (GJB1/1753)
Protein Target Info & Infographic
Gene/Protein Information For GJB1 (Source: Uniprot.org, NCBI)
Gene Name
GJB1
Full Name
Gap junction beta-1 protein
Weight
32025 MW
Superfamily
connexin family
Alternative Names
Gap junction beta-1 protein;Connexin-32;Cx32;GAP junction 28 kDa liver protein;GJB1;CX32; GJB1 CMTX, CMTX1, CX32 gap junction protein beta 1 gap junction beta-1 protein|GAP junction 28 kDa liver protein|connexin-32|gap junction protein, beta 1, 32kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJB1, check out the GJB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Connexin 32 (Gap Junction Protein) Monoclonal Antibody (M01050)
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