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Product Info Summary
| SKU: | A01050 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Connexin 32/GJB1 Antibody Picoband®
View all Connexin 32/GJB1 Antibodies
SKU/Catalog Number
A01050
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Connexin 32/GJB1 Antibody Picoband® catalog # A01050. Tested in WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Connexin 32/GJB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01050)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Connexin 32/GJB1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01050 is reactive to GJB1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
32 kDa
Calculated molecular weight
32.025kDa
Background of Connexin 32/GJB1
Gap junction beta-1 protein (GJB1), also known as connexin 32 (Cx32) is a transmembrane protein that in humans is encoded by the GJB1 gene. This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01050 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: rat kidney tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Connexin 32/GJB1 using anti-Connexin 32/GJB1 antibody (A01050).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Connexin 32/GJB1 antigen affinity purified polyclonal antibody (Catalog # A01050) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 32/GJB1 at approximately 32KD. The expected band size for Connexin 32/GJB1 is at 32KD.
Protein Target Info & Infographic
Gene/Protein Information For GJB1 (Source: Uniprot.org, NCBI)
Gene Name
GJB1
Full Name
Gap junction beta-1 protein
Weight
32.025kDa
Superfamily
connexin family
Alternative Names
Gap junction beta-1 protein; Connexin-32; Cx32; GAP junction 28 kDa liver protein; GJB1; CX32 GJB1 CMTX, CMTX1, CX32 gap junction protein beta 1 gap junction beta-1 protein|GAP junction 28 kDa liver protein|connexin-32|gap junction protein, beta 1, 32kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GJB1, check out the GJB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GJB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Connexin 32/GJB1 Antibody Picoband® (A01050)
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