Click image to see more details
Product Info Summary
| SKU: | PA2241 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-RGS9 Antibody Picoband®
SKU/Catalog Number
PA2241
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-RGS9 Antibody catalog # PA2241. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RGS9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2241)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human RGS9, different from the related mouse and rat sequences by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2241 is reactive to RGS9 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
56 kDa, 77 kDa
Calculated molecular weight
76966 MW
Background of RGS9
Regulator of G-protein signaling 9, also known as RGS9 is a human gene, which codes for a protein involved in regulation of signal transduction inside cells. This gene is mapped to 17q24.1. This gene encodes a member of the RGS family of GTPase activating proteins that function in various signaling pathways by accelerating the deactivation of G proteins. This protein is anchored to photoreceptor membranes in retinal cells and deactivates G proteins in the rod and cone phototransduction cascades. Mutations in this gene result in bradyopsia. Multiple transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA2241 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Brain Tissue, Mouse Brain Tissue, human PANC-1 whole cell
Validation Images & Assay Conditions
Click image to see more details
Anti-RGS9 antibody, PA2241, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Click image to see more details
Figure 2. Western blot analysis of RGS9 using anti-RGS9 antibody (PA2241).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS9 antigen affinity purified polyclonal antibody (Catalog # PA2241) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RGS9 at approximately 56, 77KD. The expected band size for RGS9 is at 77KD.
Protein Target Info & Infographic
Gene/Protein Information For RGS9 (Source: Uniprot.org, NCBI)
Gene Name
RGS9
Full Name
Regulator of G-protein signaling 9
Weight
76966 MW
Alternative Names
Regulator of G-protein signaling 9;RGS9;RGS9; RGS9 PERRSL, RGS9 regulator of G protein signaling 9 regulator of G-protein signaling 9|regulator of G-protein signalling 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RGS9, check out the RGS9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RGS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RGS9 Antibody Picoband® (PA2241)
Hello CJ!
No publications found for PA2241
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-RGS9 Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-RGS9 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-RGS9 Antibody Picoband®
Question
We are currently using anti-RGS9 antibody PA2241 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-07
Answer
The anti-RGS9 antibody (PA2241) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-07
