Anti-RGS9 Antibody

Product Name

Anti-RGS9 Antibody

View all RGS9 Antibodies

SKU/Catalog Number

PA2241

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-RGS9 Antibody catalog # PA2241. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-RGS9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2241)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human RGS9, different from the related mouse and rat sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2241 is reactive to RGS9 in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

56 kDa, 77 kDa

Calculated molecular weight

76966 MW

Background of RGS9

Regulator of G-protein signaling 9, also known as RGS9 is a human gene, which codes for a protein involved in regulation of signal transduction inside cells. This gene is mapped to 17q24.1. This gene encodes a member of the RGS family of GTPase activating proteins that function in various signaling pathways by accelerating the deactivation of G proteins. This protein is anchored to photoreceptor membranes in retinal cells and deactivates G proteins in the rod and cone phototransduction cascades. Mutations in this gene result in bradyopsia. Multiple transcript variants encoding different isoforms have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA2241 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Positive Control

Validation Images & Assay Conditions

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Anti-RGS9 antibody, PA2241, Western blotting
Lane 1: Rat Brain Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate

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Figure 2. Western blot analysis of RGS9 using anti-RGS9 antibody (PA2241).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PANC-1 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RGS9 antigen affinity purified polyclonal antibody (Catalog # PA2241) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. Specific bands were detected for RGS9 at approximately 56, 77KD. The expected band size for RGS9 is at 77KD.

Gene/Protein Information For RGS9 (Source: Uniprot.org, NCBI)

Alternative Names

MGC26458; PERRSMGC111763; regulator of G-protein signaling 9; regulator of G-protein signalling 9; RGS9L RGS9 PERRSL, RGS9 regulator of G protein signaling 9 regulator of G-protein signaling 9|regulator of G-protein signalling 9

For more info on RGS9, check out the RGS9 Infographic

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In this infographic, you will see the following information for RGS9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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