Anti-Complement 4d (C4d) (Acute Humoral Rejection Marker) C4A Monoclonal Antibody

Product Name

Anti-Complement 4d (C4d) (Acute Humoral Rejection Marker) C4A Monoclonal Antibody

View all C4B Antibodies

SKU/Catalog Number

M01095

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Complement 4d (C4d) (Acute Humoral Rejection Marker) C4A Monoclonal Antibody (Catalog # M01095). Tested in IF, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Complement 4d (C4d) (Acute Humoral Rejection Marker) C4A Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01095)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM545

Isotype

IgG1, kappa

Immunogen

Recombinant human Complement 4d protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M01095 is reactive to C4B in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

192785 MW

Background of C4B

This monoclonal antibody is specific to Complement 4d (C4d) and it reacts with the secreted as well as cell-bound C4d.C4d is a degradation product of the activated complement factor C4b. Complement 4b is typically activated by binding of Abs to specific target molecules. Following activation and degradation of the C4 molecule, thio-ester groups are exposed, which allow transient, covalent binding of the degradation product Complement 4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. The presence of C4d in peritubular capillaries is a key indicator for acute humoral (i.e. antibody-mediated) rejection of kidney, heart, pancreas and lung allografts. As an established marker of antibody-mediated acute renal allograft rejection and its proclivity for endothelium, this component can be detected in peritubular capillaries in chronic renal allograft rejection as well as hyperacute rejection, acute vascular rejection, acute cellular rejection, and borderline rejection. It has been shown to be a significant predictor of transplant kidney graft survival. Anti-C4d, combined with anti-C3d, can be utilized as a tool for diagnosis of allograft rejection that may warrant a prompt and aggressive anti-rejection treatment.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M01095 is guaranteed for IF, IHC Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence (1:50-1:100)
Immunohistochemistry (Formalin-fixed) (1:200-1:400 for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 7.5-8.5, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For C4B (Source: Uniprot.org, NCBI)

Alternative Names

Complement C4-A;Acidic complement C4;C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2;Complement C4 beta chain;Complement C4-A alpha chain;C4a anaphylatoxin;C4b-A;C4d-A;Complement C4 gamma chain;C4A;CO4, CPAMD2; C4B C4B112, C4B2, C4B3, C4B5, C4BD, C4B_2, C4F, CH, CO4, CPAMD3, C4B complement C4B (Chido blood group) complement C4-B|C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3|Chido form of C4|basic complement C4|complement C4B1a|complement component 4B (Chido blood group)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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