Product Info Summary
SKU: | M02226 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
View all Collagen VI alpha 1 Antibodies
SKU/Catalog Number
M02226
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody catalog # M02226. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02226)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HGG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Collagen VI
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02226 is reactive to COL6A1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
75 kDa
Calculated molecular weight
108529 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02226 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: rat pancreas tissue
Validation Images & Assay Conditions
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Western blot analysis of Collagen VI expression in Human brain lysate.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded rat pancreas, using Collagen VI Antibody.
Click image to see more details
All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For COL6A1 (Source: Uniprot.org, NCBI)
Gene Name
COL6A1
Full Name
Collagen alpha-1(VI) chain
Weight
108529 MW
Superfamily
type VI collagen family
Alternative Names
Collagen alpha-1 (VI) chain;COL6A1; COL6A1 BTHLM1, OPLL, UCHMD1 collagen type VI alpha 1 chain collagen alpha-1(VI) chain|alpha 1 (VI) chain (61 AA)|collagen VI, alpha-1 polypeptide|collagen, type VI, alpha 1|epididymis secretory sperm binding protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COL6A1, check out the COL6A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COL6A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody (M02226)
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No publications found for M02226
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Customer Q&As
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4 Customer Q&As for Anti-Collagen VI COL6A1 Rabbit Monoclonal Antibody
Question
We are currently using anti-Collagen VI Rabbit Monoclonal antibody M02226 for rat tissue, and we are well pleased with the IHC-F results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
The anti-Collagen VI Rabbit Monoclonal antibody (M02226) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-28
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta using anti-Collagen VI Rabbit Monoclonal antibody M02226. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-09
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-09
Question
Our lab want to know about to test anti-Collagen VI Rabbit Monoclonal antibody M02226 on mouse placenta for research purposes, then I may be interested in using anti-Collagen VI Rabbit Monoclonal antibody M02226 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The products we sell, including anti-Collagen VI Rabbit Monoclonal antibody M02226, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-02
Question
Is a blocking peptide available for product anti-Collagen VI Rabbit Monoclonal antibody (M02226)?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
We do provide the blocking peptide for product anti-Collagen VI Rabbit Monoclonal antibody (M02226). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-06-29