Anti-Collagen III/COL3A1 Antibody Picoband®

Product Name

Anti-Collagen III/COL3A1 Antibody Picoband®

View all Collagen III alpha 1/COL3A1 Antibodies

SKU/Catalog Number

RP1033

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Collagen III/COL3A1 Antibody catalog # RP1033. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Collagen III/COL3A1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1033)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Collagen III, different from the related mouse sequence by four amino acids, and from the related rat sequence by five amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

RP1033 is reactive to COL3A1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

39 kDa

Calculated molecular weight

138564 MW

Background of Collagen III alpha 1/COL3A1

COL3A1, also called EDS4A or Collagen alpha-1 (III), is a protein that in humans is encoded by the COL3A1 gene, it is mapped to 2q32.2. COL3A1 chain is a fibrillar-forming collagen comprising 3 alpha-1 (III) chains and is expressed in early embryos and throughout embryogenesis. In adult, COL3A1 is a major component of the extracellular matrix in a variety of internal organs and skin. COL3A1 is also a fibrous scleroprotein in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. It is involved in regulation of cortical development, and it Is the major ligand of GPR56 in the developing brain.COL3A1 binding to GPR56 can inhibit neuronal migration and activate the RhoA pathway by coupling GPR56 to GNA13 and possibly GNA12

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

RP1033 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Positive Control

WB: MCF-4 Whole Cell, COLO320 Whole Cell

Validation Images & Assay Conditions

Gene/Protein Information For COL3A1 (Source: Uniprot.org, NCBI)

Alternative Names

Collagen alpha-1 (III) chain;COL3A1; COL3A1 EDS4A, EDSVASC, PMGEDSV collagen type III alpha 1 chain collagen alpha-1(III) chain|Ehlers-Danlos syndrome type IV, autosomal dominant|alpha-1 type III collagen|alpha1 (III) collagen|collagen, fetal|collagen, type III, alpha 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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