Click image to see more details
Product Info Summary
| SKU: | M02784 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Coilin Rabbit Monoclonal Antibody
SKU/Catalog Number
M02784
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Coilin Rabbit Monoclonal Antibody catalog # M02784. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Coilin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02784)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AFDC-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Coilin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02784 is reactive to COIL in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
70 kDa, 90 kDa
Calculated molecular weight
98399 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02784 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
FC 1:100
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Coilin expression in Jurkat cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For COIL (Source: Uniprot.org, NCBI)
Gene Name
COIL
Full Name
Coilin
Weight
98399 MW
Superfamily
coilin family
Alternative Names
Cytoplasmic aconitate hydratase;Aconitase;4.2.1.3;Citrate hydro-lyase;Ferritin repressor protein;Iron regulatory protein 1;IRP1;Iron-responsive element-binding protein 1;IRE-BP 1;ACO1;IREB1; COIL CLN80, p80-coilin coilin coilin|coilin p80|p80
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on COIL, check out the COIL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for COIL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Coilin Rabbit Monoclonal Antibody (M02784)
Hello CJ!
No publications found for M02784
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Coilin Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Coilin Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Coilin Rabbit Monoclonal Antibody
Question
What sample target on Western Blot application was tested with M02784?
Verified customer
Asked: 2020-01-03
Answer
Western blot analysis of Coilin expression in Jurkat cell lysate.
Boster Scientific Support
Answered: 2020-01-16
Question
We are currently using anti-Coilin Monoclonal antibody M02784 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The anti-Coilin Monoclonal antibody (M02784) has not been validated for cross reactivity specifically with horse tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-02
Question
Is this M02784 anti-Coilin Monoclonal antibody reactive to the isotypes of COIL?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
The immunogen of M02784 anti-Coilin Monoclonal antibody is A synthesized peptide derived from human Coilin Is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-12
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Coilin Monoclonal antibody M02784. Let me know if you need anything else.
J. Kulkarni
Verified customer
Asked: 2019-05-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-23
Question
Does M02784 anti-Coilin Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
K. Jones
Verified customer
Asked: 2016-03-25
Answer
It shows on the product datasheet, M02784 anti-Coilin Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-03-25
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Coilin Monoclonal antibody M02784. Please let me know if you require anything else.
K. Parker
Verified customer
Asked: 2015-09-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-16
Question
Does anti-Coilin Monoclonal antibody M02784 work on primate WB with brain?
L. Wu
Verified customer
Asked: 2014-06-18
Answer
Our lab technicians have not validated anti-Coilin Monoclonal antibody M02784 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Coilin Monoclonal antibody M02784 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-06-18
