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Product Info Summary
| SKU: | A05401-1 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC-P, WB |
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Product info
Product Name
Anti-CNOT8 Antibody (C-term)
SKU/Catalog Number
A05401-1
Size
80 µl
Description
Boster Bio Anti-CNOT8 Antibody (C-term) (Catalog # A05401-1). Tested in WB, IF, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CNOT8 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05401-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A05401-1 is reactive to CNOT8 in Human
Reconstitution
Calculated molecular weight
33540 Da
Background of CNOT8
Ubiquitous transcription factor required for a diverse set of processes. The CCR4-NOT complex functions as general transcription regulation complex.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05401-1 is guaranteed for Flow Cytometry, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:10-1:50
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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Fluorescent confocal image of Hela cell stained with CNOT8 Antibody (C-term).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.
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CNOT8 Antibody (C-term) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the CNOT8 antibody detected the CNOT8 protein (arrow).
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Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Flow cytometric analysis of CEM cells using CNOT8 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For CNOT8 (Source: Uniprot.org, NCBI)
Gene Name
CNOT8
Full Name
CCR4-NOT transcription complex subunit 8
Weight
33540 Da
Superfamily
CAF1 family
Alternative Names
CCR4-NOT transcription complex subunit 8, CAF1-like protein, CALIFp, CAF2, CCR4-associated factor 8, Caf1b, CNOT8, CALIF, POP2 CNOT8 CAF1, CALIF, Caf1b, POP2, hCAF1 CCR4-NOT transcription complex subunit 8 CCR4-NOT transcription complex subunit 8|CAF1-like protein|CAF2|CALIFp|CCR4-associated factor 8|PGK promoter directed over production
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNOT8, check out the CNOT8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNOT8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CNOT8 Antibody (C-term) (A05401-1)
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