Product Info Summary
SKU: | A08680 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-Calponin-2 CNN2 Antibody
View all Calponin 2 Antibodies
SKU/Catalog Number
A08680
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Calponin-2 CNN2 Antibody (Catalog # A08680). Tested in WB, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calponin-2 CNN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08680)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CNN2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08680 is reactive to CNN2 in Human, Mouse
Applications
A08680 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
33697 MW
Background of Calponin 2
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Masuda H., J. Biochem. 120:415-424(1996).
Kuromitsu J., Mol. Cell. Biol. 17:707-712(1997).
Gevaert K., Nat. Biotechnol. 21:566-569(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
![a08680 western blotting analysis 1 a08680 western blotting analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a08680-western-blotting-analysis-1.jpg)
Click image to see more details
Western blot analysis of extracts from HUVEC cells, using CNN2 antibody A08680.
![a08680 immunofluorescence analysis 1 a08680 immunofluorescence analysis 1](https://www.bosterbio.com/media/catalog/product/a/0/a08680-immunofluorescence-analysis-1.jpg)
Click image to see more details
Immunofluorescence analysis of A549 cells, using CNN2 antibody A08680.
Protein Target Info & Infographic
Gene/Protein Information For CNN2 (Source: Uniprot.org, NCBI)
Gene Name
CNN2
Full Name
Calponin-2
Weight
33697 MW
Superfamily
calponin family
Alternative Names
calponin 2; Calponin H2, smooth muscle; calponin-2; Neutral calponin CNN2 calponin 2 calponin-2|calponin H2, smooth muscle|neutral calponin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CNN2, check out the CNN2 Infographic
![CNN2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CNN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calponin-2 CNN2 Antibody (A08680)
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No publications found for A08680
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Calponin-2 CNN2 Antibody
Question
Do you have a BSA free version of anti-CNN2 antibody A08680 available?
Verified Customer
Verified customer
Asked: 2020-04-16
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CNN2 antibody A08680 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-16
Question
Does anti-CNN2 antibody A08680 work for WB with platelet?
N. Singh
Verified customer
Asked: 2018-07-27
Answer
According to the expression profile of platelet, CNN2 is highly expressed in platelet. So, it is likely that anti-CNN2 antibody A08680 will work for WB with platelet.
Boster Scientific Support
Answered: 2018-07-27
Question
I was wanting to use your anti-CNN2 antibody for WB for human platelet on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human platelet identification?
Verified Customer
Verified customer
Asked: 2018-01-23
Answer
As indicated on the product datasheet, A08680 anti-CNN2 antibody has been tested for IF, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human platelet in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-23
Question
I see that the anti-CNN2 antibody A08680 works with WB, what is the protocol used to produce the result images on the product page?
B. Evans
Verified customer
Asked: 2016-09-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-09-14
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for platelet using anti-CNN2 antibody A08680. Let me know if you need anything else.
G. Johnson
Verified customer
Asked: 2016-06-23
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2016-06-23
Question
you antibody to test anti-CNN2 antibody A08680 on human platelet for research purposes, then I may be interested in using anti-CNN2 antibody A08680 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
H. Baker
Verified customer
Asked: 2014-11-10
Answer
The products we sell, including anti-CNN2 antibody A08680, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-10
Question
I have a question about product A08680, anti-CNN2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Roberts
Verified customer
Asked: 2013-06-18
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08680 anti-CNN2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-06-18