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Product Info Summary
| SKU: | A09141 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CLK4 Antibody
SKU/Catalog Number
A09141
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CLK4 Antibody (Catalog # A09141). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLK4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09141)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human CLK4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A09141 is reactive to CLK4 in Human, Mouse
Reconstitution
Calculated molecular weight
57492 MW
Background of Clk4
Dual specificity kinase acting on both serine/threonine and tyrosine-containing substrates. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex and may be a constituent of a network of regulatory mechanisms that enable SR proteins to control RNA splicing. Phosphorylates SRSF1 and SRSF3. Required for the regulation of alternative splicing of MAPT/TAU. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.
Schultz J., Genomics 71:368-370(2001).
McNally T., Protein Sci. 12:1562-1566(2003).
Kantham L., Biochem. Biophys. Res. Commun. 304:125-129(2003).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A09141 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HT-29 cells, using CLK4 antibody A09141.
Protein Target Info & Infographic
Gene/Protein Information For CLK4 (Source: Uniprot.org, NCBI)
Gene Name
CLK4
Full Name
Dual specificity protein kinase CLK4
Weight
57492 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity protein kinase CLK4;2.7.12.1;CDC-like kinase 4;CLK4; Clk4|AI987988, C85119|CDC like kinase 4|dual specificity protein kinase CLK4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLK4, check out the CLK4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLK4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLK4 Antibody (A09141)
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No publications found for A09141
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CLK4 Antibody
Question
Is a blocking peptide available for product anti-CLK4 antibody (A09141)?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
We do provide the blocking peptide for product anti-CLK4 antibody (A09141). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-18
Question
I am interested in to test anti-CLK4 antibody A09141 on human kidney for research purposes, then I may be interested in using anti-CLK4 antibody A09141 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
The products we sell, including anti-CLK4 antibody A09141, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-26
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for kidney using anti-CLK4 antibody A09141. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-21
Question
See attached the WB image, lot number and protocol we used for kidney using anti-CLK4 antibody A09141. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
Would A09141 anti-CLK4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-12-07
Answer
As indicated on the product datasheet, A09141 anti-CLK4 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-07
Question
Is this A09141 anti-CLK4 antibody reactive to the isotypes of CLK4?
Verified Customer
Verified customer
Asked: 2017-07-28
Answer
The immunogen of A09141 anti-CLK4 antibody is Synthesized peptide derived from internal of human CLK4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-07-28
Question
We are currently using anti-CLK4 antibody A09141 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2017-07-26
Answer
The anti-CLK4 antibody (A09141) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-26
