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Product Info Summary
| SKU: | M03210 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CLIC4 Rabbit Monoclonal Antibody
SKU/Catalog Number
M03210
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CLIC4 Rabbit Monoclonal Antibody catalog # M03210. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLIC4 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03210)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
30C24
Isotype
IgG
Immunogen
A synthesized peptide derived from human CLIC4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M03210 is reactive to CLIC4 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
29 kDa
Calculated molecular weight
42064 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M03210 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human 293T whole cell, human U251 whole cell, human A431 whole cell, human U20S whole cell, rat kidney tissue, rat brain tissue, mouse kidney tissue, mouse brain tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CLIC4 using anti-CLIC4 antibody (M03210).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: rat kidney tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: mouse kidney tissue lysates,
Lane 8: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CLIC4 antigen affinity purified monoclonal antibody (Catalog # M03210) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CLIC4 at approximately 29 kDa. The expected band size for CLIC4 is at 29 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CLIC4 (Source: Uniprot.org, NCBI)
Gene Name
CLIC4
Full Name
Chloride intracellular channel protein 4
Weight
42064 MW
Superfamily
chloride channel CLIC family
Alternative Names
Glutamine synthetase;GS;6.3.1.2;Glutamate decarboxylase;4.1.1.15;Glutamate--ammonia ligase;GLUL;GLNS; CLIC4 CLIC4L, H1, MTCLIC, huH1, p64H1 chloride intracellular channel 4 chloride intracellular channel protein 4|chloride intracellular channel 4 like|epididymis secretory sperm binding protein|intracellular chloride ion channel protein p64H1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLIC4, check out the CLIC4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLIC4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLIC4 Rabbit Monoclonal Antibody (M03210)
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