Product Info Summary
SKU: | A02062 |
---|---|
Size: | 100ug |
Reactive Species: | Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-IL-17F Antibody Peroxidase Conjugated
SKU/Catalog Number
A02062
Size
100ug
Form
Lyophilized
Description
Boster Bio Anti-IL-17F Antibody Peroxidase Conjugated (Catalog # A02062). Tested in WB applications. This antibody reacts with Mouse.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-IL-17F Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02062)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
Clonality
Polyclonal
Isotype
IgG
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02062 is reactive to IL17F in Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Observed Molecular Weight
42 kDa
Calculated molecular weight
98809 MW
Background of IL-17F
IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02062 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000-1:50,000
IHC: 1:500-1:2,500
WB: 1:1,000-1:5,000
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Il17f using anti-Il17f antibody (A02062).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Il17f antigen affinity purified polyclonal antibody (Catalog # A02062) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for Il17f.
Protein Target Info & Infographic
Gene/Protein Information For IL17F (Source: Uniprot.org, NCBI)
Gene Name
IL17F
Full Name
Interleukin-17F
Weight
98809 MW
Superfamily
IL-17 family
Alternative Names
IL-17F peroxidase conjugated, interleukin-17F peroxidase conjugated, Interleukin17F, Interleukin-17F, ML1, ML-1, IL17F, IL24 IL17F CANDF6, IL-17F, ML-1, ML1 interleukin 17F interleukin-17F|cytokine ML-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IL17F, check out the IL17F Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IL17F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL-17F Antibody Peroxidase Conjugated (A02062)
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Customer Q&As
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6 Customer Q&As for Anti-IL-17F Antibody Peroxidase Conjugated
Question
Will A02062 anti-IL-17F antibody Peroxidase Conjugated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
It shows on the product datasheet, A02062 anti-IL-17F antibody Peroxidase Conjugated as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-05-06
Question
Would anti-IL-17F antibody Peroxidase Conjugated A02062 work on goat WB with blood?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
Our lab technicians have not tested anti-IL-17F antibody Peroxidase Conjugated A02062 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IL-17F antibody Peroxidase Conjugated A02062 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-03
Question
Will anti-IL-17F antibody Peroxidase Conjugated A02062 work for WB with right testis?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
According to the expression profile of right testis, IL17F is highly expressed in right testis. So, it is likely that anti-IL-17F antibody Peroxidase Conjugated A02062 will work for WB with right testis.
Boster Scientific Support
Answered: 2019-09-27
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-IL-17F antibody Peroxidase Conjugated A02062. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-18
Question
Do you have a BSA free version of anti-IL-17F antibody Peroxidase Conjugated A02062 available?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL-17F antibody Peroxidase Conjugated A02062 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-06-17
Question
I am interested in to test anti-IL-17F antibody Peroxidase Conjugated A02062 on rat right testis for research purposes, then I may be interested in using anti-IL-17F antibody Peroxidase Conjugated A02062 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-08-10
Answer
The products we sell, including anti-IL-17F antibody Peroxidase Conjugated A02062, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-08-10