Anti-IL-17F Antibody Peroxidase Conjugated

IL-17F antibody

Boster Bio Anti-IL-17F Antibody Peroxidase Conjugated (Catalog # A02062). Tested in WB applications. This antibody reacts with Mouse.

Product Info Summary

SKU: A02062
Size: 100ug
Reactive Species: Mouse
Host: Rabbit
Application: WB

Product Name

Anti-IL-17F Antibody Peroxidase Conjugated

View all IL-17F Antibodies

SKU/Catalog Number

A02062

Size

100ug

Form

Lyophilized

Description

Boster Bio Anti-IL-17F Antibody Peroxidase Conjugated (Catalog # A02062). Tested in WB applications. This antibody reacts with Mouse.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-IL-17F Antibody Peroxidase Conjugated (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02062)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free, 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Clonality

Polyclonal

Isotype

IgG

Immunogen

This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL17-F protein.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A02062 is reactive to IL17F in Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Observed Molecular Weight

42 kDa

Calculated molecular weight

98809 MW

Background of IL-17F

IL17-F (also known as ML1, ML-1, IL17F, and IL24) is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angio-genesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/ GFB, and monocyte chemoattractant protein-1. IL-17F plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02062 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000-1:50,000
IHC: 1:500-1:2,500
WB: 1:1,000-1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For IL17F (Source: Uniprot.org, NCBI)

Gene Name

IL17F

Full Name

Interleukin-17F

Weight

98809 MW

Superfamily

IL-17 family

Alternative Names

IL-17F peroxidase conjugated, interleukin-17F peroxidase conjugated, Interleukin17F, Interleukin-17F, ML1, ML-1, IL17F, IL24 IL17F CANDF6, IL-17F, ML-1, ML1 interleukin 17F interleukin-17F|cytokine ML-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on IL17F, check out the IL17F Infographic

IL17F infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for IL17F: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-IL-17F Antibody Peroxidase Conjugated

Question

Will A02062 anti-IL-17F antibody Peroxidase Conjugated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

It shows on the product datasheet, A02062 anti-IL-17F antibody Peroxidase Conjugated as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-05-06

Question

Would anti-IL-17F antibody Peroxidase Conjugated A02062 work on goat WB with blood?

Verified Customer

Verified customer

Asked: 2020-04-03

Answer

Our lab technicians have not tested anti-IL-17F antibody Peroxidase Conjugated A02062 on goat. You can run a BLAST between goat and the immunogen sequence of anti-IL-17F antibody Peroxidase Conjugated A02062 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat blood in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-04-03

Question

Will anti-IL-17F antibody Peroxidase Conjugated A02062 work for WB with right testis?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

According to the expression profile of right testis, IL17F is highly expressed in right testis. So, it is likely that anti-IL-17F antibody Peroxidase Conjugated A02062 will work for WB with right testis.

Boster Scientific Support

Answered: 2019-09-27

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for right testis using anti-IL-17F antibody Peroxidase Conjugated A02062. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-18

Question

Do you have a BSA free version of anti-IL-17F antibody Peroxidase Conjugated A02062 available?

Verified Customer

Verified customer

Asked: 2019-06-17

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-IL-17F antibody Peroxidase Conjugated A02062 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-06-17

Question

I am interested in to test anti-IL-17F antibody Peroxidase Conjugated A02062 on rat right testis for research purposes, then I may be interested in using anti-IL-17F antibody Peroxidase Conjugated A02062 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2018-08-10

Answer

The products we sell, including anti-IL-17F antibody Peroxidase Conjugated A02062, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-08-10

Order DetailsPrice
A02062

100 μg

$605

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A02062
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$605.00

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