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Product Info Summary
| SKU: | A06425 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CLCNKA/Clc Ka Antibody
SKU/Catalog Number
A06425
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CLCNKA/Clc Ka Antibody (Catalog # A06425). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CLCNKA/Clc Ka Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06425)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human CLCNKA.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A06425 is reactive to CLCNKA in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
75285 MW
Background of CLCNKA
Voltage-gated chloride channel. Chloride channels have several functions including the regulation of cell volume; membrane potential stabilization, signal transduction and transepithelial transport. May be important in urinary concentrating mechanisms.
Kieferle S., Proc. Natl. Acad. Sci. U.S.A. 91:6943-6947(1994).
Gregory S.G., Nature 441:315-321(2006).
Estevez R., Nature 414:558-561(2001).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06425 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from HuvEc cells, using CLCNKA antibody A06425.
Protein Target Info & Infographic
Gene/Protein Information For CLCNKA (Source: Uniprot.org, NCBI)
Gene Name
CLCNKA
Full Name
Chloride channel protein ClC-Ka
Weight
75285 MW
Superfamily
chloride channel (TC 2.A.49) family
Alternative Names
Chloride channel protein ClC-Ka;Chloride channel Ka;ClC-K1;CLCNKA; CLCNKA CLCK1, ClC-K1, hClC-Ka chloride voltage-gated channel Ka chloride channel protein ClC-Ka|chloride channel Ka|chloride channel, kidney, A|chloride channel, voltage-sensitive Ka
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CLCNKA, check out the CLCNKA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CLCNKA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CLCNKA/Clc Ka Antibody (A06425)
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No publications found for A06425
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CLCNKA/Clc Ka Antibody
Question
Will anti-CLCNKA/Clc Ka antibody A06425 work for WB with colon?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
According to the expression profile of colon, CLCNKA is highly expressed in colon. So, it is likely that anti-CLCNKA/Clc Ka antibody A06425 will work for WB with colon.
Boster Scientific Support
Answered: 2019-10-29
Question
We are currently using anti-CLCNKA/Clc Ka antibody A06425 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?
H. Jackson
Verified customer
Asked: 2019-10-17
Answer
The anti-CLCNKA/Clc Ka antibody (A06425) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-17
Question
Is a blocking peptide available for product anti-CLCNKA/Clc Ka antibody (A06425)?
Verified Customer
Verified customer
Asked: 2019-02-19
Answer
We do provide the blocking peptide for product anti-CLCNKA/Clc Ka antibody (A06425). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-02-19
Question
Will anti-CLCNKA/Clc Ka antibody A06425 work on monkey WB with cortex of kidney?
Verified Customer
Verified customer
Asked: 2018-11-26
Answer
Our lab technicians have not tested anti-CLCNKA/Clc Ka antibody A06425 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CLCNKA/Clc Ka antibody A06425 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cortex of kidney in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-11-26
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon using anti-CLCNKA/Clc Ka antibody A06425. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-31
Question
Is this A06425 anti-CLCNKA/Clc Ka antibody reactive to the isotypes of CLCNKA?
D. Thomas
Verified customer
Asked: 2017-11-20
Answer
The immunogen of A06425 anti-CLCNKA/Clc Ka antibody is Synthesized peptide derived from C-terminal of human CLCNKA. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-11-20
Question
I see that the anti-CLCNKA/Clc Ka antibody A06425 works with WB, what is the protocol used to produce the result images on the product page?
P. Roberts
Verified customer
Asked: 2013-05-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-05-23
