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Product Info Summary
| SKU: | A01331-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CIT Antibody Picoband®
View all Citron Kinase Antibodies
SKU/Catalog Number
A01331-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CIT Antibody Picoband® catalog # A01331-2. Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CIT Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01331-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human CIT recombinant protein (Position: Q470-H1505). Human CIT shares 95.2% and 94.7% amino acid (aa) sequence identity with mouse and rat CIT, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01331-2 is reactive to CIT in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Calculated molecular weight
231.431kDa
Background of Citron Kinase
This gene encodes a serine/threonine-protein kinase that functions in cell division. Together with the kinesin KIF14, this protein localizes to the central spindle and midbody, and functions to promote efficient cytokinesis. This protein is involved in central nervous system development. Polymorphisms in this gene are associated with bipolar disorder and risk for schizophrenia. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01331-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CIT using anti-CIT antibody (A01331-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat brain tissue lysates,
Lane 2: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CIT antigen affinity purified polyclonal antibody (A01331-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CIT at approximately 231 kDa. The expected band size for CIT is at 231 kDa.
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Figure 2. Flow Cytometry analysis of SiHa cells using anti-CIT antibody (A01331-2).
Overlay histogram showing SiHa cells stained with A01331-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CIT Antibody (A01331-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CIT (Source: Uniprot.org, NCBI)
Gene Name
CIT
Full Name
Citron Rho-interacting kinase
Weight
231.431kDa
Superfamily
protein kinase superfamily
Alternative Names
CITK; citron (rho-interacting, serine/threonine kinase 21); Citron Rho-Interacting Kinase; Citron Rho-Interacting Serine/Threonine Kinase; CRIK; KIAA0949; MCPH17; Rho-interacting Serine/Threonine kinase 21; serine/threonine kinase 21; Serine/threonine-protein kinase 21; STK21 CIT CITK, CRIK, MCPH17, STK21 citron rho-interacting serine/threonine kinase citron Rho-interacting kinase|citron (rho-interacting, serine/threonine kinase 21)|serine/threonine kinase 21|serine/threonine-protein kinase 21
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CIT, check out the CIT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CIT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CIT Antibody Picoband® (A01331-2)
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