Product Info Summary
SKU: | A03963-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Rat |
Host: | Rabbit |
Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-Cip4/TRIP10 Antibody Picoband™
SKU/Catalog Number
A03963-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cip4/TRIP10 Antibody Picoband™ catalog # A03963-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Cip4/TRIP10 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03963-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cip4/TRIP10 recombinant protein (Position: M1-M182).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03963-1 is reactive to TRIP10 in Human, Rat
Applications
A03963-1 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
85 kDa
Calculated molecular weight
Background of Cip4
Cdc42-interacting protein 4 is a protein that in humans is encoded by the TRIP10 gene. TRIP10 (thyroid hormone receptor interactor 10), also known as CIP4 (Cdc42-interacting protein 4), is a novel adaptor protein which may be invloved in plasma membrane remodeling. CIP4 has been reported to negatively regulate glucose uptake by promoting GLUT4 endocytosis in muscle cells. At least five splice variants for human CIP4 have been identified, including CIP4a (CIP4), CIP4b (FELIC), CIP4c, CIPh (CIP4/2). Western blot analysis usually demonstrates a major band around 75 kDa and a weaker band about 85 kDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Validation Images & Assay Conditions
![a03963 1 trip10 primary antibodies wb testing 1 a03963 1 trip10 primary antibodies wb testing 1](https://www.bosterbio.com/media/catalog/product/a/0/a03963-1-trip10-primary-antibodies-wb-testing-1.jpg)
Click image to see more details
Figure 1. Western blot analysis of Cip4/TRIP10 using anti-Cip4/TRIP10 antibody (A03963-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat testis tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cip4/TRIP10 antigen affinity purified polyclonal antibody (Catalog # A03963-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cip4/TRIP10 at approximately 85 kDa. The expected band size for Cip4/TRIP10 is at 68 kDa.
![a03963 1 trip10 primary antibodies if testing 2 a03963 1 trip10 primary antibodies if testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a03963-1-trip10-primary-antibodies-if-testing-2.jpg)
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Figure 2. IF analysis of Cip4/TRIP10 using anti-Cip4/TRIP10 antibody (A03963-1).
Cip4/TRIP10 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-Cip4/TRIP10 Antibody (A03963-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For TRIP10 (Source: Uniprot.org, NCBI)
Gene Name
TRIP10
Full Name
Cdc42-interacting protein 4
Weight
Superfamily
FNBP1 family
Alternative Names
cdc42-interacting protein 4; CIP4STOT; HSTP; Protein Felic; Salt tolerant protein; salt tolerator; STPTRIP-10; thyroid hormone receptor interactor 10; thyroid receptor interacting protein 10; Thyroid receptor-interacting protein 10; TR-interacting protein 10 TRIP10 CIP4, HSTP, STOT, STP, TRIP-10 thyroid hormone receptor interactor 10 cdc42-interacting protein 4|TR-interacting protein 10|protein Felic|salt tolerant protein|salt tolerator|thyroid receptor-interacting protein 10
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TRIP10, check out the TRIP10 Infographic
![TRIP10 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TRIP10: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cip4/TRIP10 Antibody Picoband™ (A03963-1)
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