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Product Info Summary
| SKU: | A02671 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-CIDE-A Antibody
SKU/Catalog Number
A02671
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-CIDE-A Antibody (Catalog # A02671). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
CIDE-A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-CIDE-A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02671)
Host
Rabbit
Contents
CIDE-A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
CIDE-A antibody was raised against a 18 amino acid peptide near the carboxy terminus of human CIDE-A. The immunogen is located within the last 50 amino acids of CIDE-A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
It has no cross activity to CIDE-B.
Reactive Species
A02671 is reactive to CIDEA in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
24687 MW
Background of CIDEA
Apoptosis is related to many diseases and induced by a family of cell death receptors. Cell death signals are transduced by DD-, DED-, or CARD-containing molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase DFF40/CAD, which is chaperoned and inhibited by DFF45/ICAD. DFF45 related proteins CIDE-A and CIDE-B (for cell death-inducing DFF-like effector A and B) were recently identified. CIDE contains a new type of domain termed CIDE-N, which has high homology with the regulatory domains of DFF45/ICAD and DFF40/CAD. Expression of CIDE-A induces DNA fragmentation and activates apoptosis, which is inhibited by DFF45. CIDE-A is expressed in many tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02671 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
CIDE-A antibody can be used for detection of CIDE-A by Western blot at 2 μg/mL. An approximately 23 kDa band can be detected. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of CIDE-A in human brain tissue lysate in the absence (A) or presence (B) of peptide (A02671P) with CIDE-A antibody at 1:2000 dilution.
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Immunohistochemistry of CIDE-A in human brain tissue with CIDE-A antibody at 5 μg/mL.
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Immunofluorescence of CIDE-A in Human Brain cells with CIDE-A antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For CIDEA (Source: Uniprot.org, NCBI)
Gene Name
CIDEA
Full Name
Cell death activator CIDE-A
Weight
24687 MW
Alternative Names
CIDE-A, Cell death activator CIDE-A, Cell death-inducing DFFA-like effector A CIDEA CIDE-A cell death inducing DFFA like effector a cell death activator CIDE-A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CIDEA, check out the CIDEA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CIDEA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CIDE-A Antibody (A02671)
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