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Product Info Summary
| SKU: | A30610 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
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Product info
Product Name
Anti-CHSY2 CHPF Antibody
SKU/Catalog Number
A30610
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CHSY2 CHPF Antibody (Catalog# A30610). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHSY2 CHPF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30610)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CHSY2. Immunogen sequence location: 631-680
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30610 is reactive to CHPF in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
85467 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30610 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Western blot analysis of lysates from LOVO and K562 cells, using CHSY2 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemistry analysis of paraffin-embedded human cervix carcinoma tissue, using CHSY2 Antibody. The picture on the right is blocked with the synthesized peptide.
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Immunofluorescence analysis of MCF7 cells, using CHSY2 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CHPF (Source: Uniprot.org, NCBI)
Gene Name
CHPF
Full Name
Chondroitin sulfate synthase 2
Weight
85467 MW
Superfamily
chondroitin N-acetylgalactosaminyltransferase family
Alternative Names
CHPF;CSS2;Chondroitin sulfate synthase 2;Chondroitin glucuronyltransferase 2;Chondroitin-polymerizing factor;ChPF;Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II;N-acetylgalactosaminyl-pro Chpf|1700028N03Rik, AI414328, D1Bwg1363e|chondroitin polymerizing factor|chondroitin sulfate synthase 2|N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase II|N-acetylgalactosaminyltransferase 2|chondroitin glucuronyltransferase 2|glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase II
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHPF, check out the CHPF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHPF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHSY2 CHPF Antibody (A30610)
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No publications found for A30610
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Recommended Resources
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-CHSY2 CHPF Antibody
Question
Will anti-CHSY2 antibody A30610 work for WB with testis?
P. Mitchell
Verified customer
Asked: 2020-03-30
Answer
According to the expression profile of testis, CHPF is highly expressed in testis. So, it is likely that anti-CHSY2 antibody A30610 will work for WB with testis.
Boster Scientific Support
Answered: 2020-03-30
Question
We are currently using anti-CHSY2 antibody A30610 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-23
Answer
The anti-CHSY2 antibody (A30610) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-23
Question
I have a question about product A30610, anti-CHSY2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-11
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30610 anti-CHSY2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-11
Question
I was wanting to use your anti-CHSY2 antibody for WB for rat testis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat testis identification?
S. Anderson
Verified customer
Asked: 2017-05-05
Answer
It shows on the product datasheet, A30610 anti-CHSY2 antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-05-05
Question
We are interested in to test anti-CHSY2 antibody A30610 on rat testis for research purposes, then I may be interested in using anti-CHSY2 antibody A30610 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Mangal
Verified customer
Asked: 2015-05-28
Answer
The products we sell, including anti-CHSY2 antibody A30610, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-CHSY2 antibody A30610. Let me know if you need anything else.
T. Krishna
Verified customer
Asked: 2014-02-25
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-25
