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Product Info Summary
| SKU: | M02230 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CHRNA4 Antibody (N-Term)
View all Nicotinic Acetylcholine R alpha 4/CHRNA4 Antibodies
SKU/Catalog Number
M02230
Size
50 µl
Description
Boster Bio Anti-CHRNA4 Antibody (N-Term) (Catalog # M02230). Tested in WB application(s). This antibody reacts with Human, Mouse.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-CHRNA4 Antibody (N-Term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02230)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This CHRNA4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 176-208 amino acids from human CHRNA4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02230 is reactive to CHRNA4 in Human, Mouse
Reconstitution
Calculated molecular weight
69957 Da
Background of Nicotinic Acetylcholine R alpha 4/CHRNA4
After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane permeable to sodium ions.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02230 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
Validation Images & Assay Conditions
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All lanes : Anti-CHRNA4 Antibody (N-Term) at 1:2000 dilution
Lane 1: HepG2 whole cell lysate
Lane 2: MOLT-4 whole cell lysate
Lane 3: SH-SY5Y whole cell lysate
Lane 4: A-673 whole cell lysate
Lane 5: NCI-H1299 whole cell lysate
Lane 6: Neuro-2a whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 70 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For CHRNA4 (Source: Uniprot.org, NCBI)
Gene Name
CHRNA4
Full Name
Neuronal acetylcholine receptor subunit alpha-4
Weight
69957 Da
Superfamily
ligand-gated ion channel (TC 1.A.9) family
Alternative Names
Neuronal acetylcholine receptor subunit alpha-4, CHRNA4, NACRA4 CHRNA4 BFNC, EBN, EBN1, NACHR, NACHRA4, NACRA4 cholinergic receptor nicotinic alpha 4 subunit neuronal acetylcholine receptor subunit alpha-4|cholinergic receptor, nicotinic alpha 4|cholinergic receptor, nicotinic, alpha 4 (neuronal)|cholinergic receptor, nicotinic, alpha polypeptide 4|neuronal nicotinic acetylcholine receptor alpha-4 subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHRNA4, check out the CHRNA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHRNA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHRNA4 Antibody (N-Term) (M02230)
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