Product Info Summary
SKU: | A00311-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IHC, WB |
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Product info
Product Name
Anti-CHOP DDIT3 Monoclonal Antibody
View all GADD153/CHOP Antibodies
SKU/Catalog Number
A00311-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CHOP DDIT3 Monoclonal Antibody catalog # A00311-1. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHOP DDIT3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00311-1)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
2B1
Isotype
IgG1
Immunogen
Synthetic Peptide of CHOP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00311-1 is reactive to DDIT3 in Human, Mouse, Rat
Applications
A00311-1 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
19175 MW
Background of GADD153/CHOP
Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG.
Zhao WM, et al. (2001) EMBO J 20(9): 2315-2325.
Millard TH, et al. (2005) EMBO J 24(2): 240-250.
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Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:1000-2000
IHC, 1:100-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Pancreas Carcinoma Tissue using CHOP Mouse Monoclonal antibody diluted at 1:200.
Click image to see more details
Western Blot (WB) analysis of Mouse Liver Tissue Lysate using CHOP Mouse Monoclonal antibody diluted at 1:2000.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Stomach Carcinoma Tissue using CHOP Mouse Monoclonal antibody diluted at 1:200.
Protein Target Info & Infographic
Gene/Protein Information For DDIT3 (Source: Uniprot.org, NCBI)
Gene Name
DDIT3
Full Name
DDIT3 upstream open reading frame protein
Weight
19175 MW
Alternative Names
C/EBP-homologous protein 10; C/EBP-homologous protein; CCAAT/enhancer-binding protein homologous protein; CEBP zeta; CHOP; CHOP10 CEBPZ; CHOP10; CHOP-10; CHOPC/EBP zeta; DDIT3; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; GADD153; Growth arrest and DNA damage-inducible protein GADD153 DDIT3 AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10, CHOP10, GADD153 DNA damage inducible transcript 3 DNA damage-inducible transcript 3 protein|C/EBP zeta|CCAAT/enhancer-binding protein homologous protein|alternative DDIT3 protein|c/EBP-homologous protein 10|growth arrest and DNA damage-inducible protein GADD153
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DDIT3, check out the DDIT3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DDIT3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHOP DDIT3 Monoclonal Antibody (A00311-1)
Hello CJ!
A00311-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning
Ding Y, Zou J, Li Z, Tian J, Abdelalim S, Du F, She R, Wang D, Tan C, Wang H, Chen W, Lv D, Chang L. Plos One. 2011;6(5):E20008. Doi: 10.1371/Journal.Pone.0020008. Epub 2011 May 23. Study Of Histopathological And Molecular Changes Of Rat Kidney Un...
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Customer Q&As
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1 Customer Q&As for Anti-CHOP DDIT3 Monoclonal Antibody
Question
What is the concentration of the A00311-1?
Verified customer
Asked: 2020-01-11
Answer
The concentration of theAnti-CHOP DDIT3 Monoclonal Antibody (A00311-1) is 1 mg/ml.
Boster Scientific Support
Answered: 2020-01-11