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Product Info Summary
| SKU: | A01125-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CHD4 Antibody Picoband®
SKU/Catalog Number
A01125-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CHD4 Antibody Picoband® catalog # A01125-3. Tested in WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CHD4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01125-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human CHD4, identical to the related mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01125-3 is reactive to CHD4 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
260 kDa
Calculated molecular weight
218.005kDa
Background of CHD4
Chromodomain-helicase-DNA-binding protein 4 is an enzyme that in humans is encoded by the CHD4 gene. The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01125-3 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human U20S whole cell lysates, human HEL whole cell lysates, human K562 whole cell lysates, human MCF-7 whole cell lysates
FCM: MCF-7 cells
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CHD4 using anti-CHD4 antibody (A01125-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates,
Lane 2: human HEL whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CHD4 antigen affinity purified polyclonal antibody (Catalog # A01125-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CHD4 at approximately 260 kDa. The expected band size for CHD4 is at 218 kDa.
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Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-CHD4 antibody (A01125-3).
Overlay histogram showing MCF-7 cells stained with A01125-3 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CHD4 Antibody (A01125-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CHD4 (Source: Uniprot.org, NCBI)
Gene Name
CHD4
Full Name
Chromodomain-helicase-DNA-binding protein 4
Weight
218.005kDa
Superfamily
SNF2/RAD54 helicase family
Alternative Names
ATP-dependent helicase CHD4; CHD-4; chromodomain helicase DNA binding protein 4; chromodomain-helicase-DNA-binding protein 4; DKFZp686E06161; EC 3.6.1; EC 3.6.4.12; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA CHD4 CHD-4, Mi-2b, Mi2-BETA, SIHIWES chromodomain helicase DNA binding protein 4 chromodomain-helicase-DNA-binding protein 4|ATP-dependent helicase CHD4|Mi-2 auto 218 kDa protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHD4, check out the CHD4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHD4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHD4 Antibody Picoband® (A01125-3)
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